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Reference: Zamb TJ and Roth R (1977) Role of mitotic replication genes in chromosome duplication during meiosis. Proc Natl Acad Sci U S A 74(9):3951-5

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Abstract

In the yeast Saccharomyces cerevisiae, DNA synthesis preceding meiosis requires the expression of replication genes used during mitosis. Diploids carrying either a temperature-sensitive lesion in cdc4 (a gene required for initiation of mitotic DNA synthesis) or a lesion in cdc8 (a gene controlling mitotic polymerization) completed premeiotic DNA synthesis at a permissive but not at a restrictive temperature. The roles of cdc4 and cdc8 were evaluated by characterizing the kinetics of premeiotic DNA synthesis after a shift to a restrictive temperature. In the cdc8 diploid, DNA synthesis was immediately inhibited, consistent with a role in polymerization. In contrast, cdc4 exhibited residual DNA synthesis characteristic of an initiation function. The cdc4 gene function was completed much earlier in the meiotic cycle than the cdc8-mediated step.

Reference Type
Journal Article | Research Support, U.S. Gov't, P.H.S.
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Zamb TJ, Roth R
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