In the yeast Saccharomyces cerevisiae, DNA synthesis preceding meiosis requires the expression of replication genes used during mitosis. Diploids carrying either a temperature-sensitive lesion in cdc4 (a gene required for initiation of mitotic DNA synthesis) or a lesion in cdc8 (a gene controlling mitotic polymerization) completed premeiotic DNA synthesis at a permissive but not at a restrictive temperature. The roles of cdc4 and cdc8 were evaluated by characterizing the kinetics of premeiotic DNA synthesis after a shift to a restrictive temperature. In the cdc8 diploid, DNA synthesis was immediately inhibited, consistent with a role in polymerization. In contrast, cdc4 exhibited residual DNA synthesis characteristic of an initiation function. The cdc4 gene function was completed much earlier in the meiotic cycle than the cdc8-mediated step.
|Evidence ID||Analyze ID||Interactor||Interactor Systematic Name||Interactor||Interactor Systematic Name||Type||Assay||Annotation||Action||Modification||Phenotype||Source||Reference||Note|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Gene Ontology Term||Gene Ontology Term ID||Qualifier||Aspect||Method||Evidence||Source||Assigned On||Reference||Annotation Extension|
|Evidence ID||Analyze ID||Gene||Gene Systematic Name||Phenotype||Experiment Type||Experiment Type Category||Mutant Information||Strain Background||Chemical||Details||Reference|
|Evidence ID||Analyze ID||Regulator||Regulator Systematic Name||Target||Target Systematic Name||Experiment||Conditions||Strain||Source||Reference|