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Reference: Yang X, et al. (1992) A protein kinase substrate identified by the two-hybrid system. Science 257(5070):680-2

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Abstract

A genetic method, the two-hybrid system, was used to identify four genes encoding proteins that interact with the SNF1 protein kinase from yeast. One of the genes, SIP1, was independently isolated as a multicopy suppressor of defects caused by reduced SNF1 kinase activity, and genetic evidence supports its function in the SNF1 pathway. The SIP1 protein co-immunoprecipitated with SNF1 and was phosphorylated in vitro. Thus, the two-hybrid system, which is applicable to any cloned gene, can be used to detect physical interactions between protein kinases and functionally related substrate proteins.

Reference Type
Journal Article
Authors
Yang X, Hubbard EJ, Carlson M
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Interactor Interactor Type Assay Annotation Action Modification Phenotype Source Reference

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Gene Gene Ontology Term Qualifier Aspect Method Evidence Source Assigned On Annotation Extension Reference

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