Take our Survey

Reference: Westermann B and Neupert W (1997) Mdj2p, a novel DnaJ homolog in the mitochondrial inner membrane of the yeast Saccharomyces cerevisiae. J Mol Biol 272(4):477-83

Reference Help

Abstract


Members of the heat shock protein 70 (Hsp70) family mediate import, folding, assembly and degradation of proteins in mitochondria. The function of Hsp70 proteins is dependent on their interaction with cofactors, including members of the DnaJ protein family. The mitochondrial DnaJ homolog, Mdj1p, has been shown to cooperate with the major mitochondrial Hsp70, mt-Hsp70. We describe the identification of a second mitochondrial DnaJ homolog, Mdj2p, in the yeast Saccharomyces cerevisiae. The protein possesses an N-terminal transmembrane domain that anchors it in the mitochondrial inner membrane. The C-terminal J-domain shares 30% amino acid identity with the J-domain of Escherichia coli DnaJ and is exposed to the mitochondrial matrix. Mdj2p carries a putative internal mitochondrial targeting signal and is imported into mitochondria in a membrane potential-dependent manner. Deletion of the MDJ2 gene did not result in a detectable growth defect. Double mutants of mdj1 and mdj2 showed severe growth defects at elevated temperature, indicating a distinct overlap of the functions of Mdj1p and Mdj2p. Copyright 1997 Academic Press Limited.

Reference Type
Journal Article
Authors
Westermann B, Neupert W
Primary Lit For
Additional Lit For
Review For

Interaction Annotations


Increase the total number of rows showing on this page by using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details about experiment type and any other genes involved in the interaction.

Interactor Interactor Type Assay Annotation Action Modification Phenotype Source Reference

Gene Ontology Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table.

Gene Gene Ontology Term Qualifier Aspect Method Evidence Source Assigned On Annotation Extension Reference

Phenotype Annotations


Increase the total number of rows showing on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; filter the table using the "Filter" box at the top of the table; click on the small "i" buttons located within a cell for an annotation to view further details.

Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

Regulation Annotations


Increase the total number of rows displayed on this page using the pull-down located below the table, or use the page scroll at the table's top right to browse through the table's pages; use the arrows to the right of a column header to sort by that column; to filter the table by a specific experiment type, type a keyword into the Filter box (for example, “microarray”); download this table as a .txt file using the Download button or click Analyze to further view and analyze the list of target genes using GO Term Finder, GO Slim Mapper, SPELL, or YeastMine.

Regulator Target Experiment Assay Construct Conditions Strain Background Reference