Reference: Russell DW, et al. (1983) Nucleotide sequence of the yeast alcohol dehydrogenase II gene. J Biol Chem 258(4):2674-82

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Abstract


The complete nucleotide sequence of the glucose-repressed alcohol dehydrogenase II gene (ADR2) from yeast has been established together with its 5'- and 3'-flanking regions. The limits of the gene have been determined by reverse transcriptase sequencing of the 5'-ends and by S1 nuclease mapping of the 3'-ends of the mature ADR2 mRNA. Comparison of the alcohol dehydrogenase I gene (ADC1) sequence (Bennetzen, J. L., and Hall, B.D. (1982) J. Biol. Chem. 257, 3018-3025) with that of ADR2 indicated four regions of sequence conservation in the 5'-flanking DNAs. One of these conserved regions contains the sequence TCAAG which may function as a yeast cap sequence. The coding sequence of ADR2 is 89% homologous with that of ADC1 and exhibits a bias in its codon utilization. Evidence is presented that the intergenic region at the 3'-end of the ADR2 gene is less than 550 base pairs.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't | Research Support, U.S. Gov't, P.H.S.
Authors
Russell DW, Smith M, Williamson VM, Young ET
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