Reference: Morales MJ, et al. (1992) A 105-kDa protein is required for yeast mitochondrial RNase P activity. Proc Natl Acad Sci U S A 89(20):9875-9

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Abstract


RNase P from the mitochondria of Saccharomyces cerevisiae was purified to near homogeneity > 1800-fold with a yield of 1.6% from mitochondrial extracts. The most abundant protein in the purified fractions is, at 105 kDa, considerably larger than the 14-kDa bacterial RNase P protein subunits. Oligonucleotides designed from the amino-terminal sequence of the 105-kDa protein were used to identify and isolate the 105-kDa protein-encoding gene. Strains carrying a disruption of the gene for the 105-kDa protein are viable but respiratory deficient and accumulate mitochondrial tRNA precursors with 5' extensions. As this is the second gene known to be necessary for yeast mitochondrial RNase P activity, we have named it RPM2 (for RNase P mitochondrial).

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Journal Article | Research Support, U.S. Gov't, P.H.S.
Authors
Morales MJ, Dang YL, Lou YC, Sulo P, Martin NC
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