We have used the yeast, Saccharomyces cerevisiae, as a model system to identify a new protein component of the U3 small nucleolar ribonucleoprotein (snoRNP) and to study its role in pre-rRNA processing. Mpp10p, which we have cloned and characterized from humans, mice and yeast, is a 110 kDa protein in yeast. Antibodies to it immunoprecipitate the U3 snoRNA, indicating that it is a U3 snoRNP component. MPP10 is an essential gene. Depletion of Mpp10p by repression of MPP10 expressed from a conditional promoter causes slow growth. Analysis of pre-rRNA processing in the depleted cells indicates that Mpp10p is required for processing at the 3 U3 snoRNA-dependent sites in the pre-rRNA (A0, A1, A2). Truncations of terminal charged domains create Mpp10 proteins that confer slow growth at 30 degrees C and/or at 22 degrees C. Analysis of pre-rRNA processing in these mutants indicates a deficiency in processing at only two of the U3 snoRNA-dependent sites in the pre-rRNA (A1 and A2). Therefore truncated Mpp10 proteins are able to separate the function of the U3 snoRNP into a requirement for it in distinct processing events.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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