In the yeast Saccharomyces cerevisiae, at least ten genes are involved in excision repair of DNA damaged by UV radiation and by other agents that distort the DNA helix. Mutations in the RAD1, RAD2, RAD3, RAD4 and RAD10 genes render cells highly defective in the incision of damaged DNA, whereas mutations in the RAD7, RAD14, RAD16, RAD23 and MMS19 genes reduce the level of damage excision. This review summarizes the evidence for the involvement of these genes in excision repair and highlights the important features in the structures of the proteins encoded by the various RAD genes. The RAD3 protein has been purified and characterized in our laboratory, and it possesses single stranded DNA dependent ATPase and DNA helicase activities. The RAD3 helicase moves along the single-stranded DNA in the 5'----3' direction. We suggest that this activity plays a role in strand displacement synthesis during excision repair and in DNA replication.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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