Reference: Horecka J (1995) Localization of the FAR3 gene: genetic mapping and molecular cloning using a chromosome walk-'n'-roll strategy. Yeast 11(7):691-6

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Abstract

FAR3 is a newly-discovered yeast gene required specifically for pheromone-mediated cell cycle arrest. I have used strains harboring the far3-1 mutation to map the gene to the right arm of chromosome XIII, establishing the gene order CEN13-LYS7-MCM1-FAR3. I cloned the FAR3 gene based on its genetic map position using a strategy that combined chromosome walking and a related technique termed 'chromosome rolling'. In addition to the genetic and physical localization of FAR3, I present data that suggest corrections to the tentative map positions of VAN1 and ARG80.

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Horecka J
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