Reference: Haucke V and Schatz G (1997) Reconstitution of the protein insertion machinery of the mitochondrial inner membrane. EMBO J 16(15):4560-7

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Abstract


We have reconstituted the protein insertion machinery of the yeast mitochondrial inner membrane into proteoliposomes. The reconstituted proteoliposomes have a distinct morphology and protein composition and correctly insert the ADP/ATP carrier (AAC) and Tim23p, two multi-spanning integral proteins of the mitochondrial inner membrane. The reconstituted system requires a membrane potential, but not Tim44p or mhsp70, both of which are required for the ATP-driven translocation of proteins into the matrix. The protein insertion machinery can thus operate independently of the energy-transducing Tim44p-mhsp70 complex.

Reference Type
Journal Article | Research Support, Non-U.S. Gov't
Authors
Haucke V, Schatz G
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