Reference: Mazzei GJ, et al. (1993) A Ca(2+)-independent protein kinase C from fission yeast. J Biol Chem 268(10):7401-6

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Abstract

A protein kinase C homologue of Schizosaccharomyces pombe, pkc1+, was isolated from a genomic library by screening with the Saccharomyces cerevisiae PKC1 probe. From its primary sequence and biochemical properties, we conclude that S. pombe pkc1+ encodes a phospholipid-activated Ca(2+)-independent protein kinase, homologous to the delta/epsilon classes of mammalian protein kinase C. Gene disruption experiments show that pkc1+ is not essential for cell viability; however, overexpression of the protein leads to an abnormal cell morphology and a block in cell separation following mitosis suggestive of a role in cell division. In vitro phosphorylation experiments reveal several potential pkc1+ substrates.

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Journal Article
Authors
Mazzei GJ, Schmid EM, Knowles JK, Payton MA, Maundrell KG
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