Mannosylphosphorylation is a major oligosaccharide modification that provides negative charge in the Saccharomyces cerevisiae cell wall. Although two genes, MNN6 and MNN4, which encode a mannosylphosphate transferase and its putative positive regulator, respectively, are involved in this modification, the amount of Mnn4p has been found to be a limiting factor for mannosylphosphorylation. The level of mannosylphosphorylation increased at late-logarithmic and stationary phases of cell growth, and this was correlated to the transcriptional enhancement of MNN4. We also find that mannosylphosphate transfer to mannan is negatively regulated by the protein kinase A pathway, while the presence of 0.5 M potassium chloride enhanced MNN4 transcription. This type of transcriptional regulation is observed in many stress response genes, implying that mannosylphosphate transfer is involved in the cellular response to a variety of stresses.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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