Reference: Nguyen MM and Livingston DM (1997) Cold-sensitive rad52 alleles of yeast. Curr Genet 32(2):100-7

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Abstract


We have characterized two rad52 mutations that are cold-sensitive for growth on MMS agar. The mutations change residues 61 and 69, respectively, in the 504 amino-acid open reading frame. Neither mutation has a profound effect on mitotic crossing-over or on gene conversion. One has a severe deficiency in sporulation at all temperatures, while the other has a partial deficiency and reduced spore viability. Both mutants are retarded in growth on MMS agar by a high-copy plasmid expressing RAD51.

Reference Type
Journal Article | Research Support, U.S. Gov't, Non-P.H.S.
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Nguyen MM, Livingston DM
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