In Saccharomyces cerevisiae, the mRNA encoded by the PPR1 gene is very unstable (t1/2 = 1 min), whereas the mRNA encoded by the URA3 gene is relatively stable (t1/2 = 10 min). To identify cis-acting sequences that dictate mRNA decay rates in yeast, we have constructed PPR1/URA3 gene fusions and measured the half-lives of the resulting chimeric transcripts. The mRNA containing the URA3 coding region fused to the untranslated regions (UTR) of PPR1 decayed at a rate similar to the native PPR1 mRNA, suggesting that the instability of the PPR1 mRNA is not linked to its coding sequence. When the 5'-UTR of PPR1 was replaced by the 5'-UTR of URA3, the chimeric transcript was strongly stabilized, indicating that the 5'-UTR of PPR1 is required for the rapid decay of its mRNA. Fusion of this PPR1 5'-UTR to the URA3 coding region was sufficient to destabilize the chimeric mRNA. We conclude that the PPR1 5'-UTR contains sequence(s) that can promote rapid mRNA decay in yeast.
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Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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Site | Modification | Modifier | Source | Reference |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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