Proteins trafficking through the endoplasmic reticulum (ER) are topologically diverse. As such, multiple pathways collectively termed ER-associated degradation (ERAD) ensure that protein domains located in the lumen, membrane, and cytosol, are properly folded. The continuous nucleoplasm and cytosol also maintain a network of quality control mechanisms. These center on the Doa10, San1, and Ubr1 ubiquitin ligases. Unlike in the ER, the necessity for multiple pathways here is unclear. With all three factors localized in the nucleus, at least in part, how substrates are individually recognized is unknown. In this study, we show that the mode of biosynthesis can determine the system used for quality control. Targeting and integrating a misfolded protein to the ER membrane makes it an exclusive substrate of Doa10 whereas the soluble form of the same protein makes it a substrate of the San1/Ubr1 E3 system.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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| Evidence ID | Analyze ID | File | Description |
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