Transketolase (TK, EC 2.2.1.1), the key enzyme of the non-oxidative branch of pentose phosphate pathway of hydrocarbon transformation, plays an important role in a system of substrate rearrangement between pentose shunt and glycolysis, acting as a reversible link between the two metabolic pathways. In addition, it supplies precursors for biosyntheses of nucleotides, aromatic amino acids, and vitamins. In plants, the enzyme plays a central role in the Calvin cycle. TK catalyzes interconversion of sugar phosphates. Thiamine diphosphate (TDP) and bivalent cations serve as its cofactors. Being a typical TDP-dependent enzyme, TK is the least complex representative of this group of enzymes, and this accounts for its use as a model in studies of their structure and mechanism of action. TK is readily crystallized, this being the reason why the first crystal X-ray structure analysis of TDP-dependent enzymes was performed with a TK sample. Both the general structure of TK and the structures of its active centers have been studied in detail. In this article, we review experimental evidence of functional nonequivalence of the two active centers of TK, which are known to be identical by crystal X-ray structure analysis.
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Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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Site | Modification | Modifier | Source | Reference |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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