Saccharomyces cerevisiae erg9 mutants blocked at squalene synthase require ergosterol for growth and produce E,E-farnesol. Typically, at least half the amount of farnesol remains cell associated. Practically insoluble in water, farnesol can be extracted from production cultures of the erg9 mutants using either methanol/hexane or poly(styrene-co-divinylbenzene) beads. The first method consumes more solvents and requires centrifugation to clear an interface emulsion. The second method uses 50% less solvent and the beads can be used repeatedly for extraction. The solvent-free crude extract from the beads extraction contained higher concentration of farnesol (76-77%) than that from the solvent extraction (61-65%). Farnesol was obtained after normal-phase chromatography in high overall recovery (94%) and purity (99%).
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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