Although whole-genome sequencing of many organisms has been completed, numerous newly discovered genes are still functionally unknown. Using high-throughput data such as protein-protein interaction (PPI) information to assign putative protein function to the unknown genes has been proposed, since in many cases it is not feasible to annotate the newly discovered genes by sequence-based approaches alone. In addition to PPI data, information such as protein localization within a cell may be employed to improve protein function prediction in two ways: 1) By using such localization information as a direct indicator of protein function (e.g. nucleolus localized proteins might be involved in ribosome biogenesis), and 2) by refining noisy PPI data by localization information. In the latter case, localization information may be used to distinguish different types of PPIs: Namely, interactions between co-localized proteins (more reliable), and interactions between differently localized proteins (potentially less reliable). In this paper, we propose a probabilistic method to predict protein function from PPI data and localization information. A Bayesian network is used to model dependencies between protein function, PPI data and localization information. We showed in our cross-validation experiment that in some cases, our method (conditioning PPI data by localization information) significantly improves prediction precision, as compared to a simple Naive Bayes method that assumes PPI data and localization information are conditionally independent given protein function. Finally, we predicted 57 unknown genes as "ribosome biogenesis" proteins.
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Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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Site | Modification | Modifier | Source | Reference |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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