Saccharomyces cerevisiae uses different mechanisms to adapt to changes in environmental osmolarity. Upon hyperosmotic shock, cells first mobilize a rapid rescue system that prevents excessive loss of ions and water; then in the adaptation period they accumulate a compatible solute (glycerol). When subjected to hypoosmotic shock, they rapidly release intracellular stocks of glycerol to reduce intracellular osmolarity and prevent bursting. The plasma membrane Nha1 alkali metal cation/H+ antiporter is not important in helping the cells to survive a sudden drop in external osmolarity, but is involved in the cell response to hyperosmotic shock. For this role, its long hydrophilic C-terminus is indispensable. The capacity of the Nha1 antiporter to transport potassium is regulated by Hog1 kinase. Upon sorbitol-mediated stress, the Nha1p potassium export activity decreases in order to maintain a higher intracellular concentration of solutes. The C-terminal-less Nha1 version is not inactivated and its potassium efflux activity renders cells very sensitive to hyperosmotic shock. Taken together, our results suggest an important role of Nha1p and its C-terminus in the immediate response to hyperosmotic shock as part of the rapid rescue mechanism.
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Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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Site | Modification | Modifier | Source | Reference |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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