The PHO system is an ingenious mechanism by which the yeast Saccharomyces cerevisiae regulates the expression of a set of genes involved in phosphate metabolism in response to the change of phosphate concentrations in the environment. A key factor in this mechanism is the Pho85 kinase, which has been discovered as a negative regulator of the PHO system. One of the genes isolated in our laboratory in screening the protein kinase genes from Candida albicans was identified as a homologue (CaPHO85) of the PHO85 of S. cerevisiae, based on the following results. a) Pho85 is the polypeptide with the highest homology to CaPho85 (62% identity) among the S. cerevisiae genome sequence. b) The position of insertion of the intron is quite similar between CaPHO85 (in the 7th codon of the N-terminal MTGSSSQ) and S. cerevisiae PHO85 (in the 6th codon of the N-terminal MSSSSQ). c) The nucleotide sequences in the intron possess the consensus sequences for yeast intron: the 5-splice-site, internal, and the 3-splice-site sequence. d) CaPHO85 complemented the S. cerevisiae pho85 mutation. e) CaPho85 contains all of the consensus sequences for the ATP-binding domain and for the kinase domain found in S. cerevisiae Pho85.
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| Evidence ID | Analyze ID | Gene/Complex | Systematic Name/Complex Accession | Qualifier | Gene Ontology Term ID | Gene Ontology Term | Aspect | Annotation Extension | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Phenotype | Experiment Type | Experiment Type Category | Mutant Information | Strain Background | Chemical | Details | Reference |
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| Evidence ID | Analyze ID | Gene | Gene Systematic Name | Disease Ontology Term | Disease Ontology Term ID | Qualifier | Evidence | Method | Source | Assigned On | Reference |
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| Evidence ID | Analyze ID | Regulator | Regulator Systematic Name | Target | Target Systematic Name | Direction | Regulation of | Happens During | Regulator Type | Direction | Regulation Of | Happens During | Method | Evidence | Strain Background | Reference |
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| Site | Modification | Modifier | Source | Reference |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Allele | Assay | Annotation | Action | Phenotype | SGA score | P-value | Source | Reference | Note |
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| Evidence ID | Analyze ID | Interactor | Interactor Systematic Name | Interactor | Interactor Systematic Name | Assay | Annotation | Action | Modification | Source | Reference | Note |
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| Complement ID | Locus ID | Gene | Species | Gene ID | Strain background | Direction | Details | Source | Reference |
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| Evidence ID | Analyze ID | Dataset | Description | Keywords | Number of Conditions | Reference |
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