Reference: Kuhara S, et al. (2002) [Drug discovery based on microarray]. Nihon Yakurigaku Zasshi 120(1):47P-50P

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Abstract


Gene regulatory networks developed from full genome expression libraries from gene perturbation variant cell lines can be used to quickly and efficiently identify the molecular mechanism of action of drugs or lead compound molecules. We developed an extensive yeast gene expression library consisting of full-genome cDNA array data for over 500 yeast strains each with a single gene disruption. Using this data, combined with dose and time course expression experiments with the oral antifungal agent, we used Boolean network discovery techniques to determine the genes whose expression was most profoundly affected by this drug. Our system identified the gene as the most significantly suppressed target molecule due to exposure to the antifungal agent. This process for network based drug discovery can significantly decrease the time and resources necessary to make rational drug targeting decisions.

Reference Type
Journal Article | Review
Authors
Kuhara S, Tashiro K, Muta S
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Gene Ontology Annotations


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Gene/Complex Qualifier Gene Ontology Term Aspect Annotation Extension Evidence Method Source Assigned On Reference

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Gene Phenotype Experiment Type Mutant Information Strain Background Chemical Details Reference

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Gene Disease Ontology Term Qualifier Evidence Method Source Assigned On Reference

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Site Modification Modifier Reference

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Interactor Interactor Allele Assay Annotation Action Phenotype SGA score P-value Source Reference

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Gene Species Gene ID Strain background Direction Details Source Reference