Reference: Trilisenko LV, et al. (2002) The content and chain length of polyphosphates from vacuoles of Saccharomyces cerevisiae VKM Y-1173. Biochemistry (Mosc) 67(5):592-6

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Abstract


The content of inorganic polyphosphates (polyP) in vacuoles of the yeast Saccharomyces cerevisiae is approximately 15% of the total cellular polyP. Over 80% of the vacuole polyP are in an acid-soluble fraction. It was first established by (31)P-NMR spectroscopy that a polymeric degree (n) of two subfractions obtained by precipitation with Ba(2+) in succession at pH 4.5 and 8.2 was approximately 20 +/- 5 and 5 +/- 2 residues of ortho-phosphoric acid, respectively. Under a deficit of phosphate (P(i)) in the cultivation medium, the polyP content in vacuoles decreased approximately 7-fold with the same drastic reduction of their content in the cell. Unlike intact yeast cells, where polyP overcompensation is observed after their transfer from phosphate-free to phosphate-containing medium, the vacuoles do not show this effect. The data indicate the occurrence of special regulatory mechanisms of polyP synthesis in vacuoles differing from those in the whole cell.

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Journal Article | Research Support, Non-U.S. Gov't
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Trilisenko LV, Vagabov VM, Kulaev IS
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