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RSP5 / YER125W Overview

Standard Name
RSP5 1
Systematic Name
YER125W
SGD ID
S000000927
Aliases
SMM1 2 , MDP1 3 , MUT2 , NPI1 4 , UBY1
Feature Type
ORF , Verified
Description
NEDD4 family E3 ubiquitin ligase; regulates processes including: MVB sorting, the heat shock response, transcription, endocytosis and ribosome stability; ubiquitinates Sec23p, Sna3p, Ste4p, Nfi1p, Rpo21p and Sem1p; autoubiquitinates; deubiquitinated by Ubp2p; regulated by SUMO ligase Siz1p, in turn regulates Siz1p SUMO ligase activity; required for efficient Golgi-to-ER trafficking in COPI mutants; mutant tolerates aneuploidy; human homolog implicated in Liddle syndrome 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20
Name Description
Reverses Spt- Phenotype 21
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

Analyze Sequence

S288C only

BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation

S288C vs. other species

BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi | Fungal Alignment | Synteny Viewer

S288C vs. other strains

Strain Alignment | Variant Viewer

Protein Details

Protein

Basic information about the protein. Click "Protein Details" for further information about the protein such as abundance data, domains, shared domains with other proteins, protein sequence retrieval for various strains, sequence-based physico-chemical proterties, protein modification sites sites, and external identifiers for the protein.

Length (a.a.)
809
Mol. Weight (Da)
91810.2
Isoelectric Point
6.67
Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Summary
E3 ubiquitin ligase that conjugates ubiquitin to proteins to target them for degradation; known to ubiquitinate Ste4p; involved in regulating a variety of processes via ubiquitin-dependent catabolism including organization of the mitochondrion, multivesilular body size, initiation of the mating tip projection and actin cytoskeletal organization; positively regulates dolichol and fatty acid biosynthesis; localized throughout the cell

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated
High-Throughput
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Summary
Essential gene; conditional (ts) mutations lead to buildup of unprocessed reporter proteins normally targeted for degradation and to defects in processing of mRNA, rRNA and tRNA precursors; reduction of function causes higher sensitivity to cell wall-affecting chemicals (caffeine, Calcofluor White); repression results in opi- phenotype (overproduction and excretion of inositol in the absence of inositol and choline); overexpression causes slow growth

Classical Genetics

repressible
conditional
overexpression
unspecified
reduction of function

Large-scale Survey

reduction of function
null
overexpression
repressible
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

824 total interactions for 446 unique genes

Physical Interactions

  • Affinity Capture-MS: 156
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 66
  • Biochemical Activity: 114
  • Co-crystal Structure: 2
  • Co-fractionation: 2
  • Co-localization: 1
  • Co-purification: 1
  • Far Western: 2
  • PCA: 1
  • Protein-peptide: 122
  • Reconstituted Complex: 114
  • Two-hybrid: 34

Genetic Interactions

  • Dosage Growth Defect: 6
  • Dosage Lethality: 16
  • Dosage Rescue: 27
  • Negative Genetic: 79
  • Phenotypic Enhancement: 25
  • Phenotypic Suppression: 12
  • Positive Genetic: 14
  • Synthetic Growth Defect: 11
  • Synthetic Lethality: 5
  • Synthetic Rescue: 12
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

Regulators
6
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.

Last Updated: 2010-02-01

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.

Primary
192
Additional
100
Reviews
75

Resources

E.C. | Entrez Gene | Entrez RefSeq Protein | Search all NCBI | UniParc | UniProtKB