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TPK1 / YJL164C

Locus Overview

Standard Name
TPK1 1
Systematic Name
YJL164C
SGD ID
S000003700
Aliases
SRA3 2 , PKA1
Feature Type
ORF , Verified
Description
cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; phosphorylates and inhibits Whi3p to promote G1/S phase passage; partially redundant with Tpk2p and Tpk3p; phosphorylates pre-Tom40p, which impairs its import into mitochondria under non-respiratory conditions; TPK1 has a paralog, TPK3, that arose from the whole genome duplication 3 4 5 6 7 8
Name Description
Takashi's Protein Kinase 9
Paralog
TPK3 10
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

Location

Depicts genomic context; mouse over to view names, coordinates, and brief descriptions. Use the links to GBrowse and ORF Map for an interactive view of this region.

View in: GBrowse | ORF Map

Subfeatures

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Feature Feature Systematic Name Feature Type Relative Coordinates Coordinates Strand Coord. Version Seq. Version

Analyze Sequence

Protein Details

Protein

Basic information about the protein. Click "Protein Details" for further information about the protein such as abundance data, domains, shared domains with other proteins, protein sequence retrieval for various strains, sequence-based physico-chemical proterties, protein modification sites sites, and external identifiers for the protein.

Length (a.a.)
397
Mol. Weight (Da)
46064.1
Isoelectric Point
5.14
Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

508 total interactions for 377 unique genes

Physical Interactions

  • Affinity Capture-MS: 30
  • Affinity Capture-RNA: 1
  • Affinity Capture-Western: 12
  • Biochemical Activity: 293
  • PCA: 7
  • Reconstituted Complex: 12
  • Two-hybrid: 10

Genetic Interactions

  • Dosage Growth Defect: 6
  • Dosage Lethality: 2
  • Dosage Rescue: 24
  • Negative Genetic: 64
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 8
  • Positive Genetic: 7
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 7
  • Synthetic Rescue: 10
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

Regulators
6
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.

Last Updated: 2007-05-17

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.

Primary
117
Additional
143
Reviews
76

History

A catalog of any nomenclature and/or sequence changes, or other notes related to the annotation of the feature.

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Gene Date Note References

References

List of references used specifically on this page as citations for information in the Overview (e.g. gene names, aliases, Descriptions), Summary Paragraph, or History sections. The complete list of curated references associated with this locus can be found by clicking on the "Literature" tab for this locus.

    Resources