FLO8 / YER109C Overview
- Standard Name
- FLO8 1 2
- Systematic Name
- YER109C
- SGD ID
- SGD:S000000911
- Aliases
- PHD5 5 , YER108C , STA10 6
- Feature Type
- blocked reading frame
- Description
- Transcription factor; required for flocculation, diploid filamentous growth, and haploid invasive growth; forms a heterodimer with Mss11p that interacts with the Swi/Snf complex during transcriptional activation of FLO1, FLO11, and STA1; S. cerevisiae genome reference strain S288C contains an internal in-frame stop at codon 142, which in other strains encodes tryptophan 1 2 3 4
- Name Description
- FLOcculation
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 798
- Mol. Weight (Da)
- 86647.9
- Isoelectric Point
- 9.35
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.
View all FLO8 alleles in SGD search | YeastMine
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Sequence-specific DNA binding transcriptional activator involved in regulation of cell adhesion, flocculation, invasive growth, and starch catabolism
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in positive regulation of cell adhesion involved in single-species biofilm formation (IMP)
- involved in positive regulation of flocculation (IMP)
- involved in positive regulation of invasive growth in response to glucose limitation (IMP)
- involved in positive regulation of pseudohyphal growth (IMP, IDA)
- involved in positive regulation of starch catabolic process (IMP)
- involved in positive regulation of transcription by RNA polymerase II (IMP, IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutants have abnormal vacuolar morphology, and cannot flocculate or form biofilms, and they cannot undergo filamentous growth; overexpression interferes with chromatin silencing and increases filamentous growth
Classical Genetics
- null
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
103 total interactions for 81 unique genes
Physical Interactions
- Affinity Capture-MS: 2
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 4
- Reconstituted Complex: 3
Genetic Interactions
- Dosage Rescue: 20
- Negative Genetic: 34
- Phenotypic Enhancement: 3
- Phenotypic Suppression: 23
- Positive Genetic: 5
- Synthetic Rescue: 3
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- FLO8 encodes a transcription factor that lacks overall similarity to known transcription regulator families, although it has similarity to Mss11p. Flo8p activates transcription of genes required for filamentous growth and adhesion, such as the cell wall adhesin genes FLO1, FLO10, and FLO11, and the glucoamylase gene STA1. At the FLO11 promoter, Flo8p is a player in a complicated regulatory mechanism involving noncoding RNAs: Flo8p activates transcription of the PWR1 ncRNA, which in turn interferes with transcription of the inhibitory ICR1 ncRNA, resulting in activation of FLO11 transcription. Flo8p is required for promoter binding of Mga1p, another transcription factor that regulates filamentous growth. Flo8p activity is regulated by the cAMP-protein kinase A (PKA) pathway. Phosphorylation of Flo8p by PKA (Tpk2p) promotes Flo8p binding to FLO11 promoter.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.