RBP95 / YCR016W Overview
- Standard Name
- RBP95 1
- Systematic Name
- YCR016W
- SGD ID
- SGD:S000000609
- Feature Type
- ORF , Verified
- Description
- RNA-binding ribosome assembly factor; constituent of early pre-60S particles; genetically and physically linked to most NOP8 60s ribosome pre-assembly complex members and to ribosomal protein Rpl3p; contains two independent RNA-interacting domains; associates with helix H95 in 3' region of 25S rRNA, in close proximity to binding sites of Npa1p and Rpl3p; interacts with several snoRNAs 1
- Name Description
- rRNA-binding protein helix H95 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
- Summary
- RBP95 is located on the right arm of chromosome III between YCRCdelta7 Ty1 LTR and CWH43 GPI lipid remodelase; coding sequence is 873 nucleotides long with 6 SNPs, 3 of which cause amino acid polymorphisms
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Rbp95p is 290 amino acids long, shorter-lived, low in abundance; contains two independent RNA-interacting domains; acetylated on K10, sumoylated on K136, phosphorylated on 7 residues
- Length (a.a.)
- 290
- Mol. Weight (Da)
- 33594.3
- Isoelectric Point
- 8.36
- Median Abundance (molecules/cell)
- 3782 +/- 407
- Half-life (hr)
- 7.9
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.
View all RBP95 alleles in SGD search | YeastMine
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Nucleolar ribosome assembly factor; binds 25S rRNA and snoRNAs
Molecular Function
- Manually Curated
- enables RNA binding (IDA)
Biological Process
- Manually Curated
- involved in maturation of LSU-rRNA (IMP)
- involved in ribosomal large subunit biogenesis (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; overexpression slows growth; null mutant displays small defect in vacuolar fragmentation, sensitivity to boric acid, cycloheximide, heat, anoxia
Classical Genetics
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- Rbp95p interacts physically with proteins involved in rRNA processing; RBP95 interacts genetically with genes involved in rRNA processing
400 total interactions for 297 unique genes
Physical Interactions
- Affinity Capture-MS: 91
- Affinity Capture-RNA: 43
- Affinity Capture-Western: 18
- Biochemical Activity: 1
- Protein-peptide: 2
- Proximity Label-MS: 22
- Reconstituted Complex: 9
- Two-hybrid: 1
Genetic Interactions
- Dosage Lethality: 1
- Negative Genetic: 172
- Positive Genetic: 31
- Synthetic Growth Defect: 3
- Synthetic Lethality: 6
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 2
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.