TIF6 / YPR016C Overview


Standard Name
TIF6 1
Systematic Name
YPR016C
SGD ID
SGD:S000006220
Aliases
CDC95 3
Feature Type
ORF , Verified
Description
Constituent of 66S pre-ribosomal particles; has similarity to human translation initiation factor 6 (eIF6); may be involved in the biogenesis and or stability of 60S ribosomal subunits 2
Name Description
Translation Initiation Factor 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
245
Mol. Weight (Da)
26442.9
Isoelectric Point
4.27
Median Abundance (molecules/cell)
15404 +/- 5195
Half-life (hr)
10.8

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all TIF6 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Protein that binds ribosomal large subunit and is involved in biogenesis and maturation of LSU-ribosomal RNA (rRNA) and 5.8S rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); contributes to ribosomal subunit export from nucleus

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Essential gene; repression causes cell cycle arrest in G1; in large-scale studies, mutation decreases filamentous and invasive growth; repression reduces competitive fitness and confers resistance to cycloheximide; diploid heterozygous null mutant is sensitive to heat and verrucarin A, displays smaller cell size, and is haploinsufficient
Disease Details

Disease

Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.


Summary
Yeast TIF6 is homologous to human EIF6 and has been used to study somatic genetic rescue in Shwachman-Diamond syndrome, a leukemia predisposition disorder caused by a germline defect in ribosome assembly and characterized by neutropenia, exocrine pancreatic insufficiency, and skeletal abnormalities

Manually Curated

Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


942 total interactions for 640 unique genes

Physical Interactions

  • Affinity Capture-MS: 298
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 81
  • Biochemical Activity: 1
  • Co-crystal Structure: 3
  • Co-purification: 4
  • PCA: 6
  • Reconstituted Complex: 2
  • Two-hybrid: 1

Genetic Interactions

  • Dosage Rescue: 2
  • Negative Genetic: 442
  • Phenotypic Suppression: 2
  • Positive Genetic: 79
  • Synthetic Growth Defect: 1
  • Synthetic Lethality: 3
  • Synthetic Rescue: 12
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
7
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
30
Additional
49
Reviews
17

Resources