- Standard Name
- Systematic Name
- SGD ID
- Feature Type
DNA damage response protein kinase; required for cell-cycle arrest in response to DNA damage; activated by trans autophosphorylation when interacting with hyperphosphorylated Rad9p; also interacts with ARS1 and plays a role in initiation of DNA replication; activates the downstream kinase Dun1p; differentially senses mtDNA depletion and mitochondrial ROS; required for regulation of copper genes in response to DNA-damaging agents; relocalizes to cytosol in response to hyoxia
- Name Description
- RADiation sensitive
The S. cerevisiae Reference Genome sequence is derived from laboratory strain
S288C. Download DNA or protein sequence, view genomic context and
coordinates. Click "Sequence Details" to view all sequence information for this locus, including that
for other strains.
Basic information about the protein. Click "Protein Details" for further information about the protein
such as abundance data, domains, shared domains with other proteins, protein sequence retrieval for
various strains, sequence-based physico-chemical proterties, protein modification sites sites, and
external identifiers for the protein.
Gene Ontology Details
- Length (a.a.)
- Mol. Weight (Da)
- Isoelectric Point
GO Annotations consist of four mandatory components: a gene product, a term from one of the three
Gene Ontology (GO) controlled vocabularies
Biological Process, and
Cellular Component), a reference, and an
evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the
literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view
all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Protein serine/threonine/tyrosine kinase that binds DNA replication origins; involved in the DNA damage checkpoint, DNA replication initiation, and the biosynthesis of deoxyribonucleoside triphosphates; localizes to the nucleus and the cytosol
View computational annotations
- Manually Curated
- Manually Curated
- Manually Curated
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable
(e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background,
and a reference. In addition, annotations are classified as classical genetics or high-throughput
(e.g., large scale survey, systematic mutation set). Whenever possible, allele information and
additional details are provided. Click "Phenotype Details" to view all phenotype annotations and
evidence for this locus as well as phenotypes it shares with other genes.
- reduction of function
Interaction annotations are curated by BioGRID and include physical
or genetic interactions observed
between at least two genes. An interaction annotation is composed of the interaction type, name of the
interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a
reference, as well as other experimental details. Click "Interaction Details" to view all interaction
annotations and evidence for this locus, including an interaction visualization.
631 total interactions for 352 unique genes
- Affinity Capture-MS: 76
- Affinity Capture-Western: 51
- Biochemical Activity: 71
- Co-crystal Structure: 1
- Co-localization: 1
- Co-purification: 1
- Protein-peptide: 6
- Reconstituted Complex: 10
- Two-hybrid: 28
- Dosage Growth Defect: 15
- Dosage Lethality: 5
- Dosage Rescue: 30
- Negative Genetic: 38
- Phenotypic Enhancement: 24
- Phenotypic Suppression: 25
- Positive Genetic: 3
- Synthetic Growth Defect: 124
- Synthetic Lethality: 61
- Synthetic Rescue: 61
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the
given locus, based on experimental evidence. This evidence includes data generated through
high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO
enrichment among regulation Targets, and a regulator/target diagram for the locus.
Expression data are derived from records contained in the
Gene Expression Omnibus (GEO), and are first log2
transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result
there may be a greater number of conditions than datasets represented in a single clickable histogram
bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from
those that are up-regulated (red). Click "Expression Details" to view all expression annotations and
details for this locus, including a visualization of genes that share a similar expression pattern.
A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links
to gene names and curated GO terms are included within the Summary Paragraphs.
All manually curated literature for the specified gene, organized into topics according to their
relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details"
to view all literature information for this locus, including shared literature between genes.