MRS2 / YOR334W Overview


Standard Name
MRS2 1
Systematic Name
YOR334W
SGD ID
SGD:S000005861
Feature Type
ORF , Verified
Description
Mitochondrial inner membrane Mg(2+) channel; required for maintenance of intramitochondrial Mg(2+) concentrations at the correct level to support splicing of group II introns; similar to bacterial CorA 2 3 5
Name Description
Mitochondrial RNA Splicing 4
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
MRS2 is located in the right arm of chromosome XV between VMA4 vacuolar H+-ATPase subunit and ALA1 alanyl-tRNA synthetase, dubious ORF YOR333C overlaps the 5' end of MRS2 on the opposite strand; coding sequence is 1413 nucleotides long with 16 SNPs, 3 of which cause amino acid polymorphisms
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Mrs2p is 470 amino acids long, extremely short-lived, extremely low in abundance
Length (a.a.)
470
Mol. Weight (Da)
54221.5
Isoelectric Point
9.91
Median Abundance (molecules/cell)
738 +/- 363
Half-life (min)
46.9

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all MRS2 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Cardiolipin-binding magnesium ion transmembrane transporter of the mitochondrial inner membrane

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null displays small defect in vacuolar fragmentation, increased growth on media containing mucin as carbon source, increased resistance to the toxicity of Aβ42, sensitivity to visible light induced stress, altered chronological lifespan, decreased competitive fitness in ethanol, decreased desiccation resistance, impaired respiration, increased accumulation of unspliced forms of COB and COX1 pre-mRNAs, decreased mitochondrial Mg(2+) accumulation; overexpression enhances pseudohyphal growth
Disease Details

Disease

Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.


Summary
Yeast MRS2 is homologous to human MRS2, and has been used to study how cardiolipin deficiency leads to perturbation of mitochondrial magnesium homeostasis as a contributing factor to Barth syndrome pathology

Manually Curated

Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Mrs2p interacts physically with proteins involved in translation regulation; MRS2 interacts genetically with genes involved in transmembrane transport

235 total interactions for 177 unique genes

Physical Interactions

  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 9
  • Affinity Capture-Western: 1
  • Biochemical Activity: 1
  • Co-localization: 1
  • Two-hybrid: 7

Genetic Interactions

  • Dosage Growth Defect: 2
  • Dosage Rescue: 10
  • Negative Genetic: 177
  • Phenotypic Enhancement: 1
  • Positive Genetic: 18
  • Synthetic Growth Defect: 4
  • Synthetic Rescue: 1
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
MRS2 promoter is bound by Yap1p and Yap5p in response to heat; MRS2 transcription is regulated by Sfp1p in response to stress
Regulators
3
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
19
Additional
23
Reviews
6

Resources