MIP1 / YOR330C Overview


Standard Name
MIP1 1
Systematic Name
YOR330C
SGD ID
SGD:S000005857
Feature Type
ORF , Verified
Description
Mitochondrial DNA polymerase gamma; single subunit of mitochondrial DNA polymerase in yeast, in contrast to metazoan complex of catalytic and accessory subunits; polymorphic in yeast, petites occur more frequently in some lab strains; human ortholog POLG complements yeast mip1 mutant; mutations in human POLG associated with Alpers-Huttenlocher syndrome (AHS), progressive external ophthalmoplegia (PEO), parkinsonism, other mitochondrial diseases 2 3 4 5 6 7 8 9
Name Description
MItochondrial DNA Polymerase 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
MIP1 is located on the right arm of chromosome XV between SCD5 actin organization protein and VMA4 V-ATPase subunit; coding sequence is 3765 nucleotides long with 35 SNPs, 10 of which cause amino acid polymorphisms
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Mip1p is 1254 amino acids long, extremely long-lived, and low in abundance; contains 9 mitochondrial DNA-directed DNA-polymerase family A domains
Length (a.a.)
1254
Mol. Weight (Da)
143532.1
Isoelectric Point
9.03
Median Abundance (molecules/cell)
562 +/- 465
Half-life (hr)
>= 100

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all MIP1 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Mitochondrial DNA polymerase and 3'-5' exonuclease involved in mitochondrial DNA replication and mitochondrial DNA catabolism during nitrogen starvation

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutants grow slowly, have abnormal vacuolar morphology, accumulate glycogen, cannot respire or use various nitrogen sources, are sensitive to oxidative stress and desiccation, and have shortened lifespan and small cell size; missense mutants that correspond to human Alpers disease mutations show increased mitochondrial DNA point mutations; heterozygous diploid nulls are haploinsufficient
Disease Details

Disease

Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.


Summary
Yeast MIP1 is homologous to human POLG, and has been used to study chronic progressive external ophthalmoplegia, Alpers syndrome, progeria, and peripheral nervous system disease
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Mip1p interacts physically with proteins involved in chromatin organization; MIP1 interacts genetically with genes involved in lipid metabolism

200 total interactions for 164 unique genes

Physical Interactions

  • Affinity Capture-MS: 11
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 1
  • Two-hybrid: 1

Genetic Interactions

  • Dosage Rescue: 1
  • Negative Genetic: 118
  • Phenotypic Enhancement: 3
  • Phenotypic Suppression: 6
  • Positive Genetic: 47
  • Synthetic Growth Defect: 3
  • Synthetic Lethality: 2
  • Synthetic Rescue: 4
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
MIP1 promoter is bound by Med4p, Msn2p, Rgr1p, and Yap1p in response to heat; MIP1 transcription is regulated by Sfp1p in response to stress
Regulators
6
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
53
Additional
42
Reviews
30

Resources