HIS3 / YOR202W Overview


Standard Name
HIS3 1
Systematic Name
YOR202W
SGD ID
SGD:S000005728
Aliases
HIS8 5 , HIS10 5
Feature Type
ORF , Verified
Description
Imidazoleglycerol-phosphate dehydratase; catalyzes the sixth step in histidine biosynthesis; mutations cause histidine auxotrophy and sensitivity to Cu, Co, and Ni salts; transcription is regulated by general amino acid control via Gcn4p 1 2 3 4 5
Name Description
HIStidine 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
HIS3 is located on the right arm of chromosome XV between MRM1 ribose methylase and DED1 ATP-dependent DEAD-box RNA helicase; dubious ORF YOR230W overlaps 44 nucleotides at the 3' end of HIS3; coding sequence is 663 nucleotides long with 6 SNPs, 4 of which are silent, one of which produces an Ile/Leu polymorphism at residue 30, and one that leads to a Ser/Ile polymorphism at residue 166
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
His3p is 220 amino acids long, phosphorylated at serine 205, shares ribosomal protein S5 domain 2-like domain with proteins involved in translation regulation and rRNA processing
Length (a.a.)
220
Mol. Weight (Da)
23826.4
Isoelectric Point
6.37
Median Abundance (molecules/cell)
1640 +/- 530

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all HIS3 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Imidazoleglycerol-phosphate dehydratase involved in histidine biosynthesis

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated

Pathways


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; mutations confer histidine auxotrophy and increased sensitivity to amitrol (aminotriazole) and metal ions
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Interacts physically with proteins involved in meiosis and translation regulation; interacts genetically with genes involved in protein catabolism

60 total interactions for 59 unique genes

Physical Interactions

  • Affinity Capture-MS: 20
  • Affinity Capture-RNA: 6
  • Co-purification: 1
  • Two-hybrid: 1

Genetic Interactions

  • Dosage Rescue: 3
  • Negative Genetic: 3
  • Phenotypic Enhancement: 10
  • Phenotypic Suppression: 12
  • Synthetic Growth Defect: 2
  • Synthetic Lethality: 1
  • Synthetic Rescue: 1
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
Promoter is bound by Gcn4p, transcription is regulated by Cbf1p, and by Sfp1p in response to stress
Regulators
3
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 1999-11-10

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
43
Additional
268
Reviews
19

Resources