MSN1 / YOL116W Overview


Standard Name
MSN1 1
Systematic Name
YOL116W
SGD ID
SGD:S000005476
Aliases
FUP1 2 , HRB382 , MSS10 4 7 , PHD2 8
Feature Type
ORF , Verified
Description
Transcriptional activator; involved in regulation of invertase and glucoamylase expression, invasive growth and pseudohyphal differentiation, iron uptake, chromium accumulation, and response to osmotic stress; localizes to the nucleus; relative distribution to the nucleus increases upon DNA replication stress 1 2 3 4 5 6
Name Description
Multicopy suppressor of SNF1 mutation 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Relative distribution to the nucleus increases upon DNA replication stress
Length (a.a.)
382
Mol. Weight (Da)
43057.0
Isoelectric Point
6.69
Median Abundance (molecules/cell)
612 +/- 341

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all MSN1 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Sequence-specific DNA-binding protein involved in positive regulation of transcription in response to different stresses; localizes to both nucleus and cytoplasm

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


357 total interactions for 319 unique genes

Physical Interactions

  • Affinity Capture-MS: 4
  • Affinity Capture-RNA: 6
  • Co-localization: 1
  • Two-hybrid: 3

Genetic Interactions

  • Dosage Growth Defect: 1
  • Dosage Lethality: 1
  • Dosage Rescue: 12
  • Negative Genetic: 252
  • Phenotypic Enhancement: 2
  • Phenotypic Suppression: 8
  • Positive Genetic: 62
  • Synthetic Growth Defect: 2
  • Synthetic Haploinsufficiency: 2
  • Synthetic Rescue: 1
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
MSN1 encodes a transcriptional activator without overall similarity to known transcription factor families. Msn1p binds DNA directly and localizes to the nucleus, and a LexA-Msn1p fusion functions as a transcriptional activator. MSN1 was discovered, and named, in several different genetic screens that reveal its various roles in filamentous growth, starch metabolism, carbon utilization, iron uptake, and stress responses. It was named MSN1 (Multicopy Suppressor of SNF) for suppression of the SUC2 (invertase) expression defect of a snf1 mutant; PHD2 (PseudoHyphal Determinant) for its ability to increase pseudohyphal growth when overexpressed; MSS10 (Multicopy Suppressor of STA10) for suppression of the glucoamylase production defect conferred by an allele of STA10 (FLO8) encoding a transcriptional activator; and FUP1 (Ferric Utilization Proficient) for its positive regulatory effect on iron uptake. In response to heat and osmotic stress, Msn1p activates transcription of GPD1, CTT1, and HSP12; it also activates GPD1 transcription in response to methylglyoxal stress. It is one of many transcriptional activators of the FLO11 gene, which encodes a cell wall adhesin involved in filamentous growth and cell-cell interactions.
Regulators
4
Targets
25
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
24
Additional
33
Reviews
13

Resources