ATG3 / YNR007C Overview

Standard Name
ATG3 1
Systematic Name
APG3 2 , AUT1 3
Feature Type
ORF , Verified
E2-like enzyme; involved in autophagy and cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p regulated by acetylation by NuA4 histone acetyltransferase Esa1p while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p; Atg12p-Atg5p conjugate enhances E2 activity of Atg3p by rearranging its catalytic site 2 4 5 6 7
Name Description
AuTophaGy related 1
Sequence Details


The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.

Protein Details


Basic information about the protein. Click "Protein Details" for further information about the protein such as abundance data, domains, shared domains with other proteins, protein sequence retrieval for various strains, sequence-based physico-chemical proterties, protein modification sites sites, and external identifiers for the protein.

Length (a.a.)
Mol. Weight (Da)
Isoelectric Point
Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.

Atg8 ligase (conjugates Atg8p to phosphatidylethanolamine, PE); subunit of the Atg12-Atg5-Atg16 complex; involved in macroautophagy, cytoplasm-to-vacuole targeting (CVT) pathway, late nucleophagy, piecemeal microautophagy of nucleus, protein lipidation, autophagic vacuole assembly and mitochondrion degradation; localizes to the cytosol

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated
Phenotype Details


Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.

Non-essential gene; null mutant is unable to undergo autophagy, late nucleophagy, the CVT pathway and displays decreased mitophagy and micronucleophagy; nitrogen starved null mutant has reduced viability, abnormal nuclear morphology, and is defective in degradation of total cellular protein; null mutant has decreased competitive fitness on various media; null mutant displays decreased chronological lifespan, reduced desiccation resistance and decreased resistance to zinc treatment; null mutant has increased biofilm formation; homozygous diploid null mutant displays greatly reduced sporulation and increased glycogen accumulation; overexpression results in decreased filamentous growth
Interaction Details


Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.

141 total interactions for 103 unique genes

Physical Interactions

  • Affinity Capture-MS: 3
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 6
  • Biochemical Activity: 9
  • Co-crystal Structure: 2
  • Co-purification: 1
  • Reconstituted Complex: 14
  • Two-hybrid: 12

Genetic Interactions

  • Negative Genetic: 67
  • Phenotypic Enhancement: 1
  • Phenotypic Suppression: 2
  • Positive Genetic: 16
  • Synthetic Growth Defect: 5
Regulation Details


The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.

Expression Details


Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.

Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.

Last Updated: 2008-04-25

Literature Details


All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.