RAS2 / YNL098C Overview


Standard Name
RAS2 1
Systematic Name
YNL098C
SGD ID
SGD:S000005042
Aliases
CTN5 10 , CYR3 11 , GLC5 12 , TSL7
Feature Type
ORF , Verified
Description
GTP-binding protein; regulates nitrogen starvation response, sporulation, and filamentous growth; farnesylation and palmitoylation required for activity and localization to plasma membrane; activated by increased levels of glycolysis intermediate fructose-1,6-bisphosphate; homolog of mammalian Ras proto-oncogenes; RAS2 has a paralog, RAS1, that arose from the whole genome duplication 2 3 4 5 6
Name Description
homologous to RAS proto-oncogene
Paralog
RAS1 5
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
RAS2 has a paralog, RAS1, that arose from the whole genome duplication
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
322
Mol. Weight (Da)
34705.7
Isoelectric Point
7.4
Median Abundance (molecules/cell)
14938 +/- 3692
Half-life (hr)
8.4

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all RAS2 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
GTPase involved in the regulation of filamentous growth and regulation of transcription in response to nutrient changes; primarily localized in the plasma membrane but has been detected in the ER membrane and mitochondria

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant has a respiratory growth defect, displays enhanced efficiency of G1 arrest during nutrient depletion, increased accumulation of storage carbohydrates, decreased replicative but extended chronological lifespan, decreased filamentous and invasive growth, and an ability to sporulate on rich media; dominant hyperactive allele displays a loss of viability and decreased G1 arrest during nutrient depletion, fails to accumulate storage carbohydrates, has decreased replicative and chronological lifespan, a block in nitrogen starvation induced autophagy, reduced innate thermotolerance, enhanced pseudohyphal growth, and a reduction in sporulation; overexpression increases replicative lifespan and filamentous growth

Classical Genetics

null
unspecified
overexpression
dominant negative
activation
reduction of function
Disease Details

Disease

Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.


Summary
Yeast RAS2 is homologous to human KRAS, and has been used to study colorectal cancer

Manually Curated

Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
The ras2 null mutant is viable; the null mutant of paralog ras1 is viable; the ras2 ras1 double mutant is inviable or displays a growth defect.

774 total interactions for 486 unique genes

Physical Interactions

  • Affinity Capture-MS: 18
  • Affinity Capture-RNA: 12
  • Affinity Capture-Western: 12
  • Biochemical Activity: 8
  • Co-localization: 1
  • Co-purification: 1
  • PCA: 7
  • Proximity Label-MS: 3
  • Reconstituted Complex: 13
  • Two-hybrid: 7

Genetic Interactions

  • Dosage Growth Defect: 5
  • Dosage Lethality: 7
  • Dosage Rescue: 68
  • Negative Genetic: 311
  • Phenotypic Enhancement: 24
  • Phenotypic Suppression: 71
  • Positive Genetic: 71
  • Synthetic Growth Defect: 33
  • Synthetic Lethality: 31
  • Synthetic Rescue: 71
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
12
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 1999-03-04

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
242
Additional
319
Reviews
176

Resources