PHO84 / YML123C Overview


Standard Name
PHO84
Systematic Name
YML123C
SGD ID
SGD:S000004592
Aliases
phoT 6
Feature Type
ORF , Verified
Description
High-affinity inorganic phosphate (Pi) transporter; also low-affinity manganese transporter; regulated by Pho4p and Spt7p; mutation confers resistance to arsenate; exit from the ER during maturation requires Pho86p; cells overexpressing Pho84p accumulate heavy metals but do not develop symptoms of metal toxicity 1 2 3 4 5
Name Description
PHOsphate metabolism
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
587
Mol. Weight (Da)
64372.6
Isoelectric Point
6.41
Median Abundance (molecules/cell)
5213 +/- 1735

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all PHO84 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Inorganic phosphate transmembrane transporter involved in phosphate ion transport and polyphosphate metabolism; also has manganese ion transmembrane transporter activity and selenite:proton symporter activity involved in the transport of selenite across the plasma membrane; integral component of the plasma membrane

View computational annotations

Biological Process

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant fails to grow in low-phosphate medium, does not take up inorganic phosphate, and fails to accumulate polyphosphate; null mutant displays metal resistance; ts mutant has elevated acid phosphatase activity at elevated temperature in the presence of inorganic phosphate; in large-scale studies, null mutant has reduced competitive fitness in a variety of media and has altered resistance to various chemicals
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


292 total interactions for 236 unique genes

Physical Interactions

  • Affinity Capture-MS: 35
  • Affinity Capture-RNA: 10
  • Affinity Capture-Western: 1
  • PCA: 19
  • Protein-RNA: 1
  • Proximity Label-MS: 1

Genetic Interactions

  • Dosage Rescue: 3
  • Negative Genetic: 145
  • Phenotypic Enhancement: 10
  • Phenotypic Suppression: 22
  • Positive Genetic: 22
  • Synthetic Growth Defect: 9
  • Synthetic Lethality: 6
  • Synthetic Rescue: 8
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
23
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
94
Additional
154
Reviews
58

Resources