GSH1 / YJL101C Overview


Standard Name
GSH1 1
Systematic Name
YJL101C
SGD ID
SGD:S000003637
Feature Type
ORF , Verified
Description
Gamma glutamylcysteine synthetase; catalyzes the first step in glutathione (GSH) biosynthesis; expression induced by oxidants, cadmium, and mercury; protein abundance increases in response to DNA replication stress 1 2 3 4 5 6
Name Description
glutathione (GSH) 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
GSH1 is located in the middle of the left arm of chromosome X between tRNA-Arg and LSB6 phosphatidylinositol 4-kinase; coding sequence is 2037 nucleotides long with 17 SNPs, 5 of which lead to amino acid polymorphisms
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Gsh1p is 678 amino acids long, shorter-lived, low in abundance; acetylated on K337, ubiquitinylated on K677, phosphorylated on 4 residues; protein abundance increases in response to DNA replication stress
Length (a.a.)
678
Mol. Weight (Da)
78241.2
Isoelectric Point
6.22
Median Abundance (molecules/cell)
3495 +/- 1065
Half-life (hr)
8.3

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all GSH1 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Gamma-glutamylcysteine synthetase responsible for the first step of glutathione biosynthesis; catalyzes the conversion of cysteine and glutamate to gamma-glutamylcysteine, consuming ATP; also involved in the response to hydrogen peroxide and cadmium

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated

Pathways


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutation confers glutathione auxotrophy, slow growth and increased sensitivity to oxidative stress
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Gsh1p interacts physically with proteins involved in RNA catabolism, translation regulation, and nucleobase transport; GSH1 interacts genetically with genes involved in transcription

621 total interactions for 469 unique genes

Physical Interactions

  • Affinity Capture-MS: 10
  • Affinity Capture-RNA: 5

Genetic Interactions

  • Dosage Growth Defect: 1
  • Dosage Rescue: 3
  • Negative Genetic: 446
  • Phenotypic Enhancement: 6
  • Phenotypic Suppression: 9
  • Positive Genetic: 45
  • Synthetic Growth Defect: 37
  • Synthetic Lethality: 11
  • Synthetic Rescue: 48
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
GSH1 promoter is bound by Gcn5p, Med2p, Rfx1p, Rgr1p, Spt3p, and Xbp1p in response to heat; GSH1 transcription is regulated by Gcr1p and Rap1p; GSH1 transcription is upregulated by Yap1p and CYC8-TUP1 corepressor complex in response to metal-induced oxidative stress
Regulators
15
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2007-01-02

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
109
Additional
147
Reviews
41

Resources