RAD9 / YDR217C Overview


Standard Name
RAD9 1
Systematic Name
YDR217C
SGD ID
SGD:S000002625
Feature Type
ORF , Verified
Description
DNA damage-dependent checkpoint protein; required for cell-cycle arrest in G1/S, intra-S, and G2/M, plays a role in postreplication repair (PRR) pathway; transmits checkpoint signal by activating Rad53p and Chk1p; protects double-strand breaks from premature resection; multiple cyclin dependent kinase consensus sites and the C-terminal BRCT domain contribute to DNA damage checkpoint activation; Rad9p Chk1 Activating Domain (CAD) is phosphorylated at multiple sites by Cdc28p/Clb2p 2 3 4 5 6 7
Name Description
RADiation sensitive 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
RAD9 is located on the right arm of chromosome IV between replication origin ARS427 and sporulation gene SPR28; coding sequence is 3930 nucleotides long with 22 SNPs, 13 of which cause amino acid polymorphisms
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Rad9p is 1309 amino acids long, extremely short-lived, very low in abundance; ubiquitinylated on K1139, phosphorylated on 98 residues, 6 of them by Cdc28p-Clb2p
Length (a.a.)
1309
Mol. Weight (Da)
148357.0
Isoelectric Point
4.96
Median Abundance (molecules/cell)
1279 +/- 701
Half-life (hr)
1.7

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all RAD9 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Double-stranded DNA binding protein; involved in response to DNA damage; plays a role in the DNA damage checkpoint at G1 and intra-S phases; localizes in the chromatin

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null mutant is sensitive to DNA damaging agents, DNA synthesis and topoisomerase inhibitors; null mutant is sensitive to X-rays, gamma rays and UV radiation; null mutant displays increased chromosome instability including gross chromosomal rearrangements, but decreased reversion rate in the presence of high doses of DNA damaging agents; null mutant displays elevated levels of sumoylated Rfa1p, Rfa2p, and Rad52p after DNA damage; null mutant has a decreased duration of S phase and impaired growth on non-fermentative carbon sources, decreased oxidative stress resistance, and decreased chronological lifespan; overexpression results in a decreased rate of vegetative growth
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Rad9p interacts physically with proteins involved in regulation of cell cycle; RAD9 interacts genetically with genes involved in DNA repair

1056 total interactions for 501 unique genes

Physical Interactions

  • Affinity Capture-MS: 9
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 28
  • Biochemical Activity: 6
  • Co-localization: 2
  • Co-purification: 1
  • PCA: 1
  • Protein-peptide: 4
  • Reconstituted Complex: 10
  • Two-hybrid: 13

Genetic Interactions

  • Dosage Growth Defect: 4
  • Dosage Lethality: 2
  • Dosage Rescue: 2
  • Negative Genetic: 252
  • Phenotypic Enhancement: 285
  • Phenotypic Suppression: 106
  • Positive Genetic: 44
  • Synthetic Growth Defect: 129
  • Synthetic Lethality: 57
  • Synthetic Rescue: 96
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
RAD9 promoter is bound by Tfc7p and Yap6p in response to heat; RAD9 transcription is regulated by Sfp1p in response to stress; Rad9 protein activity is regulated by Cdc28p-Clb2p
Regulators
7
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2006-03-30

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
237
Additional
290
Reviews
125

Resources