ADR1 / YDR216W Overview


Standard Name
ADR1 1
Systematic Name
YDR216W
SGD ID
SGD:S000002624
Feature Type
ORF , Verified
Description
Carbon source-responsive zinc-finger transcription factor; required for transcription of the glucose-repressed gene ADH2, of peroxisomal protein genes, and of genes required for ethanol, glycerol, and fatty acid utilization 2 3
Name Description
Alcohol Dehydrogenase II synthesis Regulator 4
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
1323
Mol. Weight (Da)
150928.6
Isoelectric Point
6.71
Median Abundance (molecules/cell)
1576 +/- 690
Half-life (hr)
2.5

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all ADR1 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Sequence specific DNA-binding RNA polymerase II transcription factor that activates expression of genes involved in peroxisome organization, ethanol, glycerol, and fatty acid utilization

View computational annotations

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene, but heterozygous diploid shows haploinsufficiency; null mutant displays decreased competitive growth on glycerol but increased competitive growth on ethanol; null mutant has decreased resistance to palmitoleic acid and oleic acid, but increased hibernating lifespan; overexpression results in increased invasive growth
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


194 total interactions for 161 unique genes

Physical Interactions

  • Affinity Capture-MS: 44
  • Affinity Capture-RNA: 2
  • Affinity Capture-Western: 10
  • Co-localization: 2
  • Reconstituted Complex: 2
  • Two-hybrid: 8

Genetic Interactions

  • Dosage Growth Defect: 1
  • Dosage Lethality: 2
  • Dosage Rescue: 3
  • Negative Genetic: 65
  • Phenotypic Enhancement: 9
  • Phenotypic Suppression: 14
  • Positive Genetic: 23
  • Synthetic Growth Defect: 5
  • Synthetic Rescue: 4
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
ADR1 encodes a transcription factor that is a member of the C2H2 zinc finger class. Adr1p binds to promoters as a monomer in the absence of glucose to activate transcription of genes required for utilization of non-glucose carbon sources. Its targets include genes involved in catabolism of nonfermentable carbon sources such as ethanol, glycerol, and lactate (e.g., ADH2, ACS1, and GUT1), and also genes involved in peroxisome biogenesis and fatty acid utilization (e.g., POX1 and PXA1). In addition, binding of Adr1p to subtelomeric sites may affect the boundaries of silenced regions. Adr1p is part of the regulatory network controlling glucose repression and its alleviation in the absence of glucose, and as such, its expression and activity are tightly regulated by multiple glucose-response signals.
Regulators
5
Targets
20
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2005-12-12

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
119
Additional
109
Reviews
31

Resources