UGA3 / YDL170W Overview
- Standard Name
- UGA3 1 2
- Systematic Name
- YDL170W
- SGD ID
- SGD:S000002329
- Feature Type
- ORF , Verified
- Description
- Transcriptional activator for GABA-dependent induction of GABA genes; binds to DNA elements found in the promoters of target genes and increases their expression in the presence of GABA (gamma-aminobutyrate); zinc finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type; localized to the nucleus; examples of GABA genes include UGA1, UGA2, and UGA4 3 4 5 6
- Name Description
- Utilization of GAba 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Zinc finger transcription factor of the Zn(2)-Cys(6) binuclear cluster domain type
- Length (a.a.)
- 528
- Mol. Weight (Da)
- 61219.4
- Isoelectric Point
- 6.69
- Median Abundance (molecules/cell)
- 1226 +/- 227
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.
View all UGA3 alleles in SGD search | YeastMine
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Promoter proximal region, sequence-specific DNA binding protein that recruits additional transcription factors to activate genes involved in the utilization of gamma-aminobutyrate (GABA)
View computational annotations
Molecular Function
- Manually Curated
- enables DNA-binding transcription activator activity, RNA polymerase II-specific (IDA)
- enables RNA polymerase II cis-regulatory region sequence-specific DNA binding (IDA)
- enables RNA polymerase II sequence-specific DNA-binding transcription factor recruiting activity (IDA, IGI)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in gamma-aminobutyric acid catabolic process (IMP)
- involved in nitrogen catabolite activation of transcription from RNA polymerase II promoter (IMP)
- involved in nitrogen utilization (IMP)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- null
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
91 total interactions for 85 unique genes
Physical Interactions
- Affinity Capture-MS: 1
- Affinity Capture-RNA: 5
- Co-localization: 1
- Two-hybrid: 1
Genetic Interactions
- Negative Genetic: 63
- Phenotypic Suppression: 1
- Positive Genetic: 11
- Synthetic Growth Defect: 8
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- UGA3 encodes a transcription factor that is a member of the C6 zinc finger class, containing a DNA binding domain also known as the Zn2Cys6 binuclear zinc cluster or zinc knuckle. Uga3p binds as a homodimer to UAS-GABA sequences in the promoters of target genes involved in uptake and utilization of the non-preferred nitrogen source gamma-aminobutyrate (GABA), such as UGA1, encoding GABA transaminase, and UGA4, encoding GABA permease. Transcriptional activation by Uga3p is promoted by GABA, probably because a conformational change upon GABA binding to Uga3p enhances its DNA-binding ability. Uga3p can activate transcription independently under artificial conditions (when overexpressed), but under physiological conditions it is one of several transcription factors involved in nitrogen source utilization (including Dal82p, Stp1p, and Stp2p) whose function depends on cooperation with the transcription factor Dal81p. The involvement of Dal81p in pathways for utilization of several different nitrogen sources allows the cell to integrate the disparate nutritional signals and to induce different pathways according to availability of each source. Binding of Uga3p to target promoters, and recruitment of the transcription coactivators SAGA and Mediator, is promoted by Dal81p. The mechanism by which Dal81p promotes Uga3p function probably involves physical interaction between the two transcription factors, since it does not require the DNA-binding domain of Dal81p.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.