GET3 / YDL100C Overview
- Standard Name
- GET3 1
- Systematic Name
- YDL100C
- SGD ID
- SGD:S000002258
- Aliases
- ARR4 2
- Feature Type
- ORF , Verified
- Description
- Guanine nucleotide exchange factor for Gpa1p; amplifies G-protein signaling; subunit of the GET complex involved in ATP-dependent Golgi-to-ER trafficking; binds as a dimer to the transmembrane domain of tail-anchored proteins in the cytosol, shielding them from aqueous solvent until the Get1p/Get2p heterodimer releases the substrate and inserts it into the ER membrane; acts as a chaperone under ATP-depleted oxidative stress conditions; protein abundance increases under DNA replication stress 1 2 3 4 5 6 7 8 9 10
- Name Description
- Guided Entry of Tail-anchored proteins 3
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- Subunit of the GET complex; binds as a dimer to the transmembrane domain of tail-anchored proteins in the cytosol; protein abundance increases under DNA replication stress
- Length (a.a.)
- 354
- Mol. Weight (Da)
- 39337.1
- Isoelectric Point
- 4.61
- Median Abundance (molecules/cell)
- 17300 +/- 7743
- Half-life (hr)
- 13.1
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.
View all GET3 alleles in SGD search | YeastMine
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Guanyl-nucleotide exchange factor involved in SRP-independent endoplasmic reticulum protein-membrane targeting and cis-Golgi to rough endoplasmic reticulum transport; subunit of GET comp;ex; localizes to cytosol and ER
View computational annotations
Molecular Function
- Manually Curated
- enables ATP hydrolysis activity (IDA)
- enables guanyl-nucleotide exchange factor activity (IDA)
- enables protein folding chaperone (IDA)
- enables unfolded protein binding (IDA)
- enables zinc ion binding (RCA)
Biological Process
- Manually Curated
- involved in cellular response to oxidative stress (IDA)
- involved in pheromone-dependent signal transduction involved in conjugation with cellular fusion (IMP)
- involved in post-translational protein targeting to endoplasmic reticulum membrane (IDA)
- involved in response to heat (IMP)
- involved in response to metal ion (IMP)
- involved in retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum (IDA, IMP, IGI)
- involved in tail-anchored membrane protein insertion into ER membrane (IMP)
Cellular Component
- Manually Curated
- located in cytosol (HDA)
- located in endoplasmic reticulum (HDA)
- part of GET complex (IPI)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- overexpression
- null
- chemical compound accumulation: increased
- heat sensitivity: increased
- mating efficiency: decreased
- mating response: decreased
- metal resistance: decreased
- mitochondrial morphology: abnormal
- oxidative stress resistance: decreased
- protein secretion: increased
- protein/peptide accumulation: abnormal
- protein/peptide accumulation: increased
- protein/peptide distribution: abnormal
- resistance to chemicals: decreased
- respiratory growth: decreased
- viable
- reduction of function
- dominant negative
- null
- acid pH resistance: decreased
- chronological lifespan: decreased
- competitive fitness: decreased
- competitive fitness: increased
- desiccation resistance: decreased
- haploinsufficient
- heat sensitivity: increased
- lipid particle morphology: abnormal
- mitotic recombination: increased
- oxidative stress resistance: decreased
- protein transport: abnormal
- protein/peptide accumulation: increased
- resistance to chemicals: decreased
- resistance to chemicals: increased
- starvation resistance: decreased
- vegetative growth: decreased rate
- viable
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
993 total interactions for 480 unique genes
Physical Interactions
- Affinity Capture-MS: 49
- Affinity Capture-RNA: 7
- Affinity Capture-Western: 19
- Biochemical Activity: 1
- Co-crystal Structure: 14
- Co-fractionation: 2
- Co-localization: 4
- Co-purification: 2
- FRET: 3
- PCA: 5
- Protein-peptide: 1
- Proximity Label-MS: 1
- Reconstituted Complex: 26
- Two-hybrid: 39
Genetic Interactions
- Dosage Growth Defect: 2
- Negative Genetic: 595
- Phenotypic Enhancement: 71
- Phenotypic Suppression: 34
- Positive Genetic: 84
- Synthetic Growth Defect: 21
- Synthetic Lethality: 9
- Synthetic Rescue: 4
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 4
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.