CDC14 / YFR028C Overview


Standard Name
CDC14 1
Systematic Name
YFR028C
SGD ID
SGD:S000001924
Aliases
OAF3 44
Feature Type
ORF , Verified
Description
Protein phosphatase required for mitotic exit; required for rDNA segregation, cytokinesis, meiosis I spindle disassembly, environmental stress response, autophagy during meiosis; downregulates microautophagy induction after TORC1 inactivation; held in nucleolus by Cdc55p in early meiosis, liberated by FEAR and Mitotic Exit Network in anaphase; sequestered in metaphase II, released upon entry into anaphase II; human homolog CDC14A can complement thermosensitivity of cdc14-1 mutant 2 3 4 5 7 8 9 10 11 12 13 14
Name Description
Cell Division Cycle 6
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
CDC14 is located on the right arm of chromosome VI between ECO1 acetyltransferase and SUP6 tRNA-Tyr; coding sequence is 1656 nucleotides long with 6 SNPs, 2 of which cause Ser/Thr polymorphisms
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Cdc14p is 551 amino acids long, shorter-lived, low in abundance; sumoylated on K203, phosphorylated on 29 residues
Length (a.a.)
551
Mol. Weight (Da)
61909.4
Isoelectric Point
8.09
Median Abundance (molecules/cell)
3030 +/- 681
Half-life (hr)
8.7

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all CDC14 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Phosphatase involved in mitotic exit, chromosome segregation, meiotic spindle disassembly, stress response and cytokinesis; subunit of the RENT complex; localizes to the nucleolus and spindle pole body

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated

Complex

Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Essential gene; conditional mutations cause cell cycle arrest in late anaphase with large-budded cells; overexpression results in slow growth
Disease Details

Disease

Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.


Summary
Yeast CDC14 is homologous to human CDC14A and CDC14B, and has been used to study vascular disease, colorectal carcinoma, and autosomal recessive nonsyndromic deafness 32
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Cdc14p interacts physically with proteins involved in mitotic cell cycle; CDC14 interacts genetically with genes involved in mitotic cell cycle

931 total interactions for 560 unique genes

Physical Interactions

  • Affinity Capture-MS: 238
  • Affinity Capture-RNA: 5
  • Affinity Capture-Western: 66
  • Biochemical Activity: 41
  • Co-crystal Structure: 6
  • Co-localization: 6
  • Co-purification: 1
  • PCA: 2
  • Protein-peptide: 3
  • Reconstituted Complex: 25
  • Two-hybrid: 27

Genetic Interactions

  • Dosage Lethality: 8
  • Dosage Rescue: 38
  • Negative Genetic: 312
  • Phenotypic Enhancement: 29
  • Phenotypic Suppression: 30
  • Positive Genetic: 24
  • Synthetic Growth Defect: 22
  • Synthetic Lethality: 24
  • Synthetic Rescue: 24
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
CDC14 promoter is bound by Fkh1p and Gcn4p; CDC14 promoter is bound by Med2p in response to heat; Cdc14 protein activity is regulated by Cdc28p
Regulators
4
Targets
4
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2010-05-18

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
219
Additional
198
Reviews
153

Resources