RSP5 / YER125W Overview


Standard Name
RSP5 1
Systematic Name
YER125W
SGD ID
SGD:S000000927
Aliases
UBY1 , MDP1 28 , MUT2 29 , NPI1 30 , SMM1 31 , HPG1 32
Feature Type
ORF , Verified
Description
NEDD4 family E3 ubiquitin ligase; regulates processes including: MVB sorting, the heat shock response, transcription, endocytosis and ribosome stability; ubiquitinates Sec23p, Sna3p, Ste4p, Nfi1p, Rpo21p, Sem1p, Dip5p and Put4p; autoubiquitinates; deubiquitinated by Ubp2p; regulated by SUMO ligase Siz1p, in turn regulates Siz1p SUMO ligase activity; required for efficient Golgi-to-ER trafficking in COPI mutants; mutant tolerates aneuploidy; human homolog implicated in Liddle syndrome 2 3 4 5 6 7 9 10 11 12 13 14 15 16 17 18 19 20
Name Description
Reverses Spt- Phenotype 8
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
809
Mol. Weight (Da)
91810.2
Isoelectric Point
6.67
Median Abundance (molecules/cell)
6335 +/- 1139
Half-life (hr)
7.8

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all RSP5 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
E3 ubiquitin ligase that conjugates ubiquitin to proteins to target them for degradation; known to ubiquitinate Ste4p; involved in regulating a variety of processes via ubiquitin-dependent catabolism including organization of the mitochondrion, multivesilular body size, initiation of the mating tip projection and actin cytoskeletal organization; positively regulates dolichol and fatty acid biosynthesis; localized throughout the cell

View computational annotations

Biological Process

Manually Curated

Cellular Component

Manually Curated

Complex

Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Essential gene; conditional (ts) mutations lead to buildup of unprocessed reporter proteins normally targeted for degradation and to defects in processing of mRNA, rRNA and tRNA precursors; reduction of function causes higher sensitivity to cell wall-affecting chemicals (caffeine, Calcofluor White); repression results in opi- phenotype (overproduction and excretion of inositol in the absence of inositol and choline); overexpression causes slow growth
Disease Details

Disease

Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.


Summary
Yeast RSP5 is orthologous to human NEDD4 and has been used to study Liddle syndrome

Manually Curated

Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


1978 total interactions for 1258 unique genes

Physical Interactions

  • Affinity Capture-MS: 176
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 86
  • Biochemical Activity: 158
  • Co-crystal Structure: 2
  • Co-fractionation: 2
  • Co-localization: 2
  • Co-purification: 1
  • Far Western: 2
  • PCA: 2
  • Protein-peptide: 125
  • Reconstituted Complex: 131
  • Two-hybrid: 35

Genetic Interactions

  • Dosage Growth Defect: 6
  • Dosage Lethality: 17
  • Dosage Rescue: 29
  • Negative Genetic: 978
  • Phenotypic Enhancement: 29
  • Phenotypic Suppression: 20
  • Positive Genetic: 129
  • Synthetic Growth Defect: 22
  • Synthetic Lethality: 6
  • Synthetic Rescue: 16
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
6
Targets
9
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2010-02-01

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
245
Additional
116
Reviews
119

Resources