GET2 / YER083C Overview
- Standard Name
- GET2 1
- Systematic Name
- YER083C
- SGD ID
- SGD:S000000885
- Aliases
- HUR2 7 , RMD7 2
- Feature Type
- ORF , Verified
- Description
- Subunit of the GET complex; involved in insertion of tail-anchored proteins proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division; Get1p and Get2p form an aqueous channel for protein translocation and insertion into the membrane 1 2 3 4 5 6
- Name Description
- Guided Entry of Tail-anchored proteins 3
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 285
- Mol. Weight (Da)
- 31500.7
- Isoelectric Point
- 9.96
- Median Abundance (molecules/cell)
- 3871 +/- 1188
- Half-life (hr)
- 8.7
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.
View all GET2 alleles in SGD search | YeastMine
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- Protein-membrane adaptor involved in mitophagy, integral ER membrane protein localization and cis-Golgi to rough endoplasmic reticulum transport; subunit of the GET complex; localizes to ER
View computational annotations
Molecular Function
- Manually Curated
- contributes to membrane insertase activity (IMP)
- enables protein transmembrane transporter activity (IDA)
- enables protein-membrane adaptor activity (IGI)
Biological Process
- Manually Curated
- involved in establishment of protein localization to endoplasmic reticulum membrane (IDA)
- involved in mitophagy (IMP)
- involved in retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum (IDA, IMP, IGI)
- involved in tail-anchored membrane protein insertion into ER membrane (IGI)
Cellular Component
- Manually Curated
- located in endoplasmic reticulum (HDA)
- is active in endoplasmic reticulum membrane (IDA)
- part of GET complex (IGI)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
- Summary
- Non-essential gene; null mutant shows defects in protein retention in ER and is sensitive to elevated temperatures, shows abnormal mitochondrial morphology, defective respiratory growth, sensitivity to antifungal drugs, cell wall-affecting drugs, oxidative stress; homozygous diploid is unable to sporulate
Classical Genetics
- null
- heat sensitivity: increased
- innate thermotolerance: increased
- metal resistance: decreased
- mitochondrial morphology: abnormal
- protein/peptide accumulation: abnormal
- protein/peptide accumulation: increased
- resistance to chemicals: decreased
- resistance to enzymatic treatment: decreased
- respiratory growth: decreased
- RNA accumulation: decreased
- null
- alkaline pH resistance: decreased
- auxotrophy
- chemical compound accumulation: abnormal
- chemical compound accumulation: decreased
- chemical compound accumulation: increased
- chronological lifespan: decreased
- colony sectoring: increased
- competitive fitness: decreased
- endoplasmic reticulum morphology: abnormal
- heat sensitivity: increased
- metal resistance: decreased
- mitochondrial morphology: abnormal
- oxidative stress resistance: decreased
- protein transport: abnormal
- resistance to chemicals: decreased
- resistance to chemicals: increased
- respiratory growth: decreased rate
- sporulation: absent
- toxin resistance: decreased
- utilization of carbon source: decreased rate
- utilization of nitrogen source: decreased rate
- vegetative growth: decreased rate
- viable
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
2323 total interactions for 1083 unique genes
Physical Interactions
- Affinity Capture-MS: 11
- Affinity Capture-RNA: 4
- Co-crystal Structure: 4
- Co-fractionation: 1
- Co-localization: 2
- FRET: 1
- PCA: 1
- Reconstituted Complex: 5
Genetic Interactions
- Dosage Growth Defect: 2
- Negative Genetic: 1378
- Phenotypic Enhancement: 161
- Phenotypic Suppression: 150
- Positive Genetic: 254
- Synthetic Growth Defect: 256
- Synthetic Lethality: 89
- Synthetic Rescue: 4
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Regulators
- 9
- Targets
- 0
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.