URA3 / YEL021W Overview


Standard Name
URA3 1
Systematic Name
YEL021W
SGD ID
SGD:S000000747
Feature Type
ORF , Verified
Description
Orotidine-5'-phosphate (OMP) decarboxylase; catalyzes the sixth enzymatic step in the de novo biosynthesis of pyrimidines, converting OMP into uridine monophosphate (UMP); converts 5-FOA into 5-fluorouracil, a toxic compound 1 2 3 4 5 6
Name Description
URAcil requiring 1 7
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
URA3 is located on the left arm of Chromosome V between the GEA2 guanine nucleotide exchange factor and TIM9 mitochondrial protein; there are 4 SNPs in URA3, one of which leads to an S/F amino acid polymorphism at residue 81
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Ura3p is 267 amino acids long, and undergoes various post-translational modifications including phosphorylation, sumoylation, and ubiquitinylation, on 17 residues
Length (a.a.)
267
Mol. Weight (Da)
29239.8
Isoelectric Point
7.45
Median Abundance (molecules/cell)
13482 +/- 5677

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all URA3 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Orotidine-5'-phosphate decarboxylase involved in the 'de novo' biosynthesis of pyrimidine nucleobases; localized to the cytosol

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated

Pathways


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Null mutant is auxotrophic for uracil, and display increased resistance to ureidosuccinic acid and 5-fluoroorotic acid (5-FOA), and show increased acetic acid sensitivity
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Interacts physically with proteins involved in translation regulation and RNA catabolism; interacts genetically with genes involved in amino acid metabolism

25 total interactions for 23 unique genes

Physical Interactions

  • Affinity Capture-MS: 8
  • Affinity Capture-RNA: 5
  • Co-crystal Structure: 1
  • Proximity Label-MS: 2
  • Two-hybrid: 2

Genetic Interactions

  • Dosage Rescue: 1
  • Synthetic Growth Defect: 4
  • Synthetic Lethality: 2
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
Promoter bound by Fkh1p and Fkh2p, and by Yap6p in response to heat; transcription is regulated by Cin5p
Regulators
4
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2005-11-17

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
105
Additional
388
Reviews
23

Resources