SRB6 / YBR253W Overview


Standard Name
SRB6 1
Systematic Name
YBR253W
SGD ID
SGD:S000000457
Aliases
MED22 4
Feature Type
ORF , Verified
Description
Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation 1 2 3
Name Description
Suppressor of RNA polymerase B 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
121
Mol. Weight (Da)
13854.9
Isoelectric Point
4.32
Median Abundance (molecules/cell)
3080 +/- 1054

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all SRB6 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Component of the mediator complex involved in the initiation of basal RNA polymerase II transcription

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated

Complex

Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


336 total interactions for 205 unique genes

Physical Interactions

  • Affinity Capture-MS: 116
  • Affinity Capture-RNA: 7
  • Affinity Capture-Western: 17
  • Co-crystal Structure: 1
  • Co-localization: 2
  • Co-purification: 8
  • PCA: 2
  • Reconstituted Complex: 7
  • Two-hybrid: 6

Genetic Interactions

  • Dosage Rescue: 1
  • Negative Genetic: 147
  • Positive Genetic: 12
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 3
  • Synthetic Rescue: 2
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
SRB6 (also called MED22) encodes a component of the head module of the Mediator transcriptional regulation complex. The Mediator complex is highly conserved from yeast to human, and is a central player in the transcriptional regulation of protein-coding genes, being required for nearly all transcription by RNA polymerase II (RNAPII). Mediator bridges activators and the general transcription machinery. The core Mediator complex contains three distinct head, middle, and tail modules. Other subunits of the head module are encoded by MED6, MED8, MED11, SRB2/MED20, SRB4/MED17, SRB5/MED18, and ROX3/MED19. The head module binds weakly to a minimal preinitiation complex (PIC) consisting of RNAPII, general initiation factors TFIIF, TFIIB and TBP, and promoter DNA to stimulate basal transcription. Mediator interacts physically with diverse DNA-binding transactivators to communicate transcription signals to RNAPII and general initiation factors to turn transcription on or off. Mediator also has important functions in post-initiation steps, such as recruiting and coordinating the activities of RNAPII elongation factors.
Regulators
2
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
27
Additional
50
Reviews
36

Resources