MEC1 / YBR136W Overview


Standard Name
MEC1 1
Systematic Name
YBR136W
SGD ID
SGD:S000000340
Aliases
ESR1 2 , SAD3 31 , RAD31 32
Feature Type
ORF , Verified
Description
Genome integrity checkpoint protein and PI kinase superfamily member; Mec1p and Dun1p function in same pathway to regulate dNTP pools and telomere length; signal transducer required for cell cycle arrest and transcriptional responses to damaged or unreplicated DNA; facilitates replication fork progression and regulates P-body formation under replication stress; promotes interhomolog recombination by phosphorylating Hop1p; associates with shortened, dysfunctional telomeres 1 2 3 4 5 6 7 8 9 10 11 12
Name Description
Mitosis Entry Checkpoint 1
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
2368
Mol. Weight (Da)
273363.7
Isoelectric Point
8.45
Median Abundance (molecules/cell)
224 +/- 204
Half-life (hr)
7.5

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all MEC1 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Protein serine/threonine kinase essential for DNA replication, DNA repair and telomere maintenance; localizes to the nucleus and mitochondria

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated

Complex

Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.


Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Essential gene; in an SML1 mutant background the null mutant displays decreased resistance to UV light, ionizing radiation, and DNA replication inhibitors that are also DNA damaging agents; null mutant inhibits phosphorylation of Rad26p, Cdc13p, and Ddc1p, and increases sumoylation of Rfa1p, Rfa2p, and Rad52p; reduction-of-function mutant has decreased G1 arrest, reduced chronological lifespan and increased apoptosis following nutrient depletion; reduction-of-function mutant has elevated mitotic recombination and decreased telomere length; overexpression results in reduced vegetative growth; heterozygous diploid null mutant has elevated chromosomal instability
Disease Details

Disease

Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.


Summary
Yeast MEC1 is homologous to human ATM and ATR, and has been used to study ataxia telangiectasia, Seckel syndrome, and carcinogenesis
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


806 total interactions for 381 unique genes

Physical Interactions

  • Affinity Capture-MS: 70
  • Affinity Capture-RNA: 3
  • Affinity Capture-Western: 30
  • Biochemical Activity: 43
  • Co-crystal Structure: 2
  • Co-localization: 2
  • Protein-peptide: 1
  • Reconstituted Complex: 8
  • Two-hybrid: 7

Genetic Interactions

  • Dosage Growth Defect: 2
  • Dosage Lethality: 15
  • Dosage Rescue: 23
  • Negative Genetic: 146
  • Phenotypic Enhancement: 100
  • Phenotypic Suppression: 72
  • Positive Genetic: 22
  • Synthetic Growth Defect: 112
  • Synthetic Lethality: 41
  • Synthetic Rescue: 107
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
6
Targets
5
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2006-03-24

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
342
Additional
331
Reviews
228

Resources