TEC1 / YBR083W Overview
- Standard Name
- TEC1 1
- Systematic Name
- YBR083W
- SGD ID
- SGD:S000000287
- Aliases
- ROC1 6
- Feature Type
- ORF , Verified
- Description
- Transcription factor targeting filamentation genes and Ty1 expression; Ste12p activation of most filamentation gene promoters depends on Tec1p and Tec1p transcriptional activity is dependent on its association with Ste12p; binds to TCS elements upstream of filamentation genes, which are regulated by Tec1p/Ste12p/Dig1p complex; competes with Dig2p for binding to Ste12p/Dig1p; positive regulator of chronological life span; TEA/ATTS DNA-binding domain family member 1 2 3 4 5
- Name Description
- Transposon Enhancement Control 1
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Summary
- TEA/ATTS DNA-binding domain family member
- Length (a.a.)
- 486
- Mol. Weight (Da)
- 55158.0
- Isoelectric Point
- 7.75
- Median Abundance (molecules/cell)
- 1151 +/- 621
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.
View all TEC1 alleles in SGD search | YeastMine
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- DNA-binding RNA polymerase II transcription factor involved in the regulation of pseudohyphal growth, and transposition; subunit of a complex with Ste12p and Dig1p that regulates transcription of mating genes
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in cellular response to stress (IDA)
- involved in invasive growth in response to glucose limitation (IMP)
- involved in positive regulation of pseudohyphal growth (IDA)
- involved in positive regulation of transcription by RNA polymerase II (IDA)
- involved in pseudohyphal growth (IMP)
Cellular Component
- Manually Curated
- located in nucleus (IDA)
- part of Tec1p-Ste12p-Dig1p complex (IDA)
Complex
Macromolecular complex annotations are imported from the Complex Portal. These annotations have been derived from physical molecular interaction evidence extracted from the literature and cross-referenced in the entry, or by curator inference from information on homologs in closely related species or by inference from scientific background.
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
- overexpression
- null
- budding: delayed
- chronological lifespan: decreased
- colony appearance: abnormal
- filamentous growth: absent
- invasive growth: absent
- protein/peptide distribution: abnormal
- pseudohyphal growth: absent
- pseudohyphal growth: decreased
- RNA accumulation: decreased
- subcellular morphology: abnormal
- transposable element transposition: decreased
- unspecified
- null
- biofilm formation: decreased
- chronological lifespan: increased
- colony appearance: abnormal
- competitive fitness: decreased
- filamentous growth: decreased
- invasive growth: absent
- oxidative stress resistance: decreased
- protein activity: increased
- resistance to chemicals: decreased
- RNA accumulation: decreased
- stress resistance: decreased
- utilization of carbon source: decreased rate
- utilization of nitrogen source: decreased rate
- viable
- overexpression
Large-scale Survey
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
212 total interactions for 136 unique genes
Physical Interactions
- Affinity Capture-MS: 18
- Affinity Capture-RNA: 6
- Affinity Capture-Western: 15
- Biochemical Activity: 2
- Co-localization: 1
- Co-purification: 1
- Protein-RNA: 1
- Reconstituted Complex: 12
- Two-hybrid: 2
Genetic Interactions
- Dosage Rescue: 7
- Negative Genetic: 96
- Phenotypic Enhancement: 7
- Phenotypic Suppression: 18
- Positive Genetic: 21
- Synthetic Growth Defect: 1
- Synthetic Rescue: 4
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- TEC1 encodes a helix-turn-helix transcription factor of the homeodomain family involved in the regulation of genes involved in the invasive/pseudohyphal growth cascade. Tec1p belongs to the TEA domain (TEAD) family of regulators that control cellular development in many eukaryotes, and recognizes a motif called the TEA/ATTS consensus sequence (TCS), 5'-RCATTCCY-3'. Tec1p and Ste12p can physically interact and are a model for studying the mechanisms of combinatorial and promoter-specific target gene control. The association of a pheromone-responsive element (PRE), containing the consensus sequence 5'-TGAAACA-3' which is recognized by Ste12p, and a TCS has been designated a filamentous responsive element (FRE) and allows binding of a Tec1p-Ste12p heterodimer to activate gene expression. However, Tec1p-Ste12p heterodimers can also regulate target genes via single TCS elements, with Tec1p providing the DNA binding and Ste12p the transcriptional activation. Tec1p can also activate target genes independently of Ste12p. Tec1p is required for FLO11 expression and for vegetative adhesion. The FLO11 promoter contains both FREs and TCS elements and is bound by Tec1p and Ste12p, though Tec1p is also able to activate FLO11 expression by without Ste12p. During vegetative growth, Dig1p and Dig2p directly inhibit the transcriptional activity of the Tec1p-Ste12p heterodimer. In response to pheromone, the inhibitory functions of Dig1p and Dig2p are relieved by phosphorylation through the mating-specific MAPKs Fus3p and Kss1p. Fus3p also phosphorylates Tec1p, which induces its degradation by the proteasome and abrogating FLO11 expression, thereby maintaining signaling specificity during the pheromone response.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.