FUS3 / YBL016W Overview


Standard Name
FUS3 1
Systematic Name
YBL016W
SGD ID
SGD:S000000112
Aliases
DAC2 28
Feature Type
ORF , Verified
Description
Mitogen-activated serine/threonine protein kinase involved in mating; phosphoactivated by Ste7p; substrates include Ste12p, Far1p, Bni1p, Sst2p; inhibits invasive growth during mating by phosphorylating Tec1p, promoting its; inhibits recruitment of Ste5p, Cdc42p-mediated asymmetry and mating morphogenesis 2 3 4 5 6 7 8
Name Description
cell FUSion 9
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
FUS3 is located on the left arm of chromosome II near the centromere and between PEP1 type I transmembrane sorting receptor and ACH1 CoA transferase; coding sequence is 1062 nucleotides long with 7 SNPs, 2 of which cause amino acid polymorphisms
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Fus3p is 353 amino acids long, short-lived, low in abundance; phosphorylated on 8 residues
Length (a.a.)
353
Mol. Weight (Da)
40770.5
Isoelectric Point
7.13
Median Abundance (molecules/cell)
4800 +/- 1651
Half-life (hr)
4.4

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all FUS3 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
MAP kinase involved in pheromone-dependent signal transduction during mating; has a role in invasive growth in response to glucose limitation and negative regulation of MAPK cascade; localized to both the nucleus and mating projection tip

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; isolated based on a bilateral cell fusion defect; null mutant has reduced mating efficiency and is unable to undergo pheromone induced G1 arrest; overexpression increases pheromone sensitivity; null mutant displays decreased autophagy and endocytosis; null mutant is hyperfilamentous and disrupts the nuclear localization of Sks1p and Ksp1p after induction of filamentous growth
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Fus3p interacts physically with proteins involved in chromatin organization; FUS3 interacts genetically with genes involved in transcription

645 total interactions for 438 unique genes

Physical Interactions

  • Affinity Capture-MS: 37
  • Affinity Capture-RNA: 4
  • Affinity Capture-Western: 26
  • Biochemical Activity: 108
  • Co-crystal Structure: 2
  • Co-fractionation: 1
  • Co-localization: 2
  • Co-purification: 1
  • FRET: 7
  • PCA: 2
  • Reconstituted Complex: 31
  • Two-hybrid: 23

Genetic Interactions

  • Dosage Growth Defect: 3
  • Dosage Rescue: 4
  • Negative Genetic: 146
  • Phenotypic Enhancement: 25
  • Phenotypic Suppression: 29
  • Positive Genetic: 172
  • Synthetic Growth Defect: 10
  • Synthetic Haploinsufficiency: 1
  • Synthetic Lethality: 2
  • Synthetic Rescue: 9
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
FUS3 promoter is bound by Fhl1p, Med2p, and Put3p in response to heat; FUS3 transcription is regulated by Sfp1p in response to stress; FUS3 transcription is upregulated by Sir2p, Sir3p, and Sir4p during mitotic cell cycle; Fus3 protein activity is autoregulated
Regulators
13
Targets
4
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2007-07-04

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
158
Additional
237
Reviews
108

Resources