PAI3 / YMR174C Overview


Standard Name
PAI3 1
Systematic Name
YMR174C
SGD ID
SGD:S000004786
Aliases
IA3 6
Feature Type
ORF , Verified
Description
Cytoplasmic proteinase A (Pep4p) inhibitor; dependent on Pbs2p and Hog1p protein kinases for osmotic induction; intrinsically unstructured, N-terminal half becomes ordered in the active site of proteinase A upon contact 2 3 4 5
Name Description
Proteinase A Inhibitor 4
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
PAI3 is located on the right arm of chromosome XIII between DDR48 damage-response protein and SIP18 hydrophilin; coding sequence is 207 nucleotides long with one SNP that causes an Val/Ile polymorphism at residue 25
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Summary
Pai3p is 68 residues long, of average half-life, low in abundance; acetylated on K59, phosphorylated on S15 and S35, sumoylated on 4 lysines
Length (a.a.)
68
Mol. Weight (Da)
7707.6
Isoelectric Point
7.69
Median Abundance (molecules/cell)
3787 +/- 1418
Half-life (hr)
10.1

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all PAI3 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Endopeptidase inhibitor involved in vacuolar protein catabolism; localizes to both cytoplasm and nucleus

View computational annotations

Molecular Function

Manually Curated

Biological Process

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Non-essential gene; null displays increased resistance to toxicity of Aβ42, impaired use of glutamine as nitrogen source, sensitivity to amiodarone and amitrole, decreased competitive fitness; heterozygous null is haploproficient
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
Pai3p interacts physically with proteins involved in RNA catabolism and regulation of cell cycle; PAI3 interacts genetically with genes involved in lipid metabolism

100 total interactions for 97 unique genes

Physical Interactions

  • Affinity Capture-MS: 1
  • Affinity Capture-RNA: 4
  • PCA: 1
  • Protein-peptide: 4
  • Reconstituted Complex: 1

Genetic Interactions

  • Negative Genetic: 68
  • Positive Genetic: 20
  • Synthetic Rescue: 1
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Summary
PAI3 promoter is bound by Cha4p, Spn1p, and Sup7p in response to heat, and by Fkh2p in reponse to starvation; PAI3 transcription is downregulated by Ixr1p, upregulated by Msn2p/Msn4p; PAI3 transcription is upregulated by Ixr1p in response to hypoxia
Regulators
7
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
18
Additional
19
Reviews
1

Resources