MIG2 / YGL209W Overview
- Standard Name
- MIG2 1
- Systematic Name
- YGL209W
- SGD ID
- SGD:S000003177
- Aliases
- MLZ1 5
- Feature Type
- ORF , Verified
- Description
- Zinc finger transcriptional repressor; cooperates with Mig1p in glucose-induced gene repression; under low glucose conditions relocalizes to mitochondrion, where it interacts with Ups1p, antagonizes mitochondrial fission factor Dnm1p, indicative of a role in mitochondrial fusion or regulating morphology; regulates filamentous growth in response to glucose depletion; activated in stochastic pulses of nuclear localization in response to low glucose 1 2 3 4
- Name Description
- Multicopy Inhibitor of GAL gene expression
- Paralog
- MIG3
- Comparative Info
-
Sequence
The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.
Analyze Sequence
S288C only
BLASTN | BLASTP | Design Primers | Restriction Fragment Map | Restriction Fragment Sizes | Six-Frame Translation
S288C vs. other species
BLASTN vs. fungi | BLASTP at NCBI | BLASTP vs. fungi
S288C vs. other strains
Protein
Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.
- Length (a.a.)
- 382
- Mol. Weight (Da)
- 42062.9
- Isoelectric Point
- 10.35
- Median Abundance (molecules/cell)
- 2274 +/- 1128
Alleles
Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.
View all MIG2 alleles in SGD search | YeastMine
Gene Ontology
GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.
- Summary
- RNA polymerase II proximal promoter sequence-specific DNA binding protein involved in negative and positive regulation by RNA polymerase II; localizes to mitochondria and nucleus
View computational annotations
Molecular Function
- Manually Curated
Biological Process
- Manually Curated
- involved in carbon catabolite repression of transcription from RNA polymerase II promoter by glucose (IDA, IGI, IMP)
- involved in negative regulation of transcription by RNA polymerase II (IMP, IGI)
- involved in positive regulation of filamentous growth of a population of unicellular organisms in response to starvation (IGI)
- involved in positive regulation of transcription by RNA polymerase II (IMP, IGI)
Cellular Component
- Manually Curated
- located in mitochondrion (IDA)
- located in nucleus (HDA, IDA)
Phenotype
Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.
Classical Genetics
Interaction
Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.
- Summary
- The mig2 null mutant is viable; the null mutant of paralog mig3 is viable; the mig2 mig3 double mutant has not been annotated for phenotype.
134 total interactions for 110 unique genes
Physical Interactions
- Affinity Capture-RNA: 5
- Affinity Capture-Western: 9
- Biochemical Activity: 5
- Co-purification: 1
- PCA: 1
- Protein-RNA: 2
- Reconstituted Complex: 2
- Two-hybrid: 4
Genetic Interactions
- Negative Genetic: 77
- Phenotypic Enhancement: 11
- Phenotypic Suppression: 7
- Positive Genetic: 6
- Synthetic Growth Defect: 2
- Synthetic Lethality: 1
- Synthetic Rescue: 1
Regulation
The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.
- Summary
- MIG2 encodes a Cys2His2 zinc-finger transcription factor of the beta-beta-alpha family. Mig2p binds 5'-CCCCRSV-3' motifs. Like Mig1p, Mig2p represses transcription of genes encoding enzymes for utilization of the sugars maltose, sucrose, or galactose when glucose is present. Mig2p participates in glucose repression by targeting a subset of Mig1p-repressed genes, and responds to higher glucose concentrations than Mig1p. The MIG2 gene is induced by glucose, and is transcriptionally regulated by the Rgt2/Snf3-Rgt1 pathway. In high-glucose conditions, Mig2p is localized in the nucleus where it cooperates with Mig1p in glucose-mediated transriptional repression. Under glucose depletion or oxidative stress, Mig2p accumulates in mitochondria and plays a role in mitochondrial fusion control. Nucleo-mitochondrial distribution of Mig2p is regulated by nutritional and oxidative stress.
Expression
Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.
Literature
All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.