HYP2 / YEL034W Overview


Standard Name
HYP2 1
Systematic Name
YEL034W
SGD ID
SGD:S000000760
Aliases
eIF5A , eIF-5A 11 , TIF51A 11
Feature Type
ORF , Verified
Description
Translation elongation factor eIF-5A; required for translation of proteins containing polyproline stretches; structural homolog of bacterial EF-P; undergoes an essential hypusination modification; HYP2 has paralog ANB1 that arose from whole genome duplication; in humans EIF5A variants cause a disorder characterized by variable combinations of developmental delay, microcephaly, micrognathia and dysmorphism; human EIF5A complements inviability of yeast hyp2 anb1 double null mutant 2 3 4 5 6 7 8 9 10
Name Description
HYPusine-containing protein 1
Paralog
ANB1 8
Comparative Info
Sequence Details

Sequence

The S. cerevisiae Reference Genome sequence is derived from laboratory strain S288C. Download DNA or protein sequence, view genomic context and coordinates. Click "Sequence Details" to view all sequence information for this locus, including that for other strains.


Summary
HYP2 is located on the left arm of chromosome V between uncharacterized gene UTR5 and uncharacterized gene MTC7; dubious ORF YEL034C-A completely overlaps HYP2 on the opposite strand; coding sequence is 474 nucleotides long with 3 SNPs, one of which causes an Asp/Glu polymorphism at residue 122; HYP2 has paralog ANB1 from whole genome duplication
Protein Details

Protein

Basic sequence-derived (length, molecular weight, isoelectric point) and experimentally-determined (median abundance, median absolute deviation) protein information. Click "Protein Details" for further information about the protein such as half-life, abundance, domains, domains shared with other proteins, protein sequence retrieval for various strains, physico-chemical properties, protein modification sites, and external identifiers for the protein.


Length (a.a.)
157
Mol. Weight (Da)
17103.4
Isoelectric Point
4.56
Median Abundance (molecules/cell)
50245 +/- 22813
Half-life (hr)
9.1

Alleles

Curated mutant alleles for the specified gene, listed alphabetically. Click on the allele name to open the allele page. Click "SGD search" to view all alleles in search results. Click "YeastMine" to view all alleles in YeastMine.


View all HYP2 alleles in SGD search | YeastMine

Gene Ontology Details

Gene Ontology

GO Annotations consist of four mandatory components: a gene product, a term from one of the three Gene Ontology (GO) controlled vocabularies (Molecular Function, Biological Process, and Cellular Component), a reference, and an evidence code. SGD has manually curated and high-throughput GO Annotations, both derived from the literature, as well as computational, or predicted, annotations. Click "Gene Ontology Details" to view all GO information and evidence for this locus as well as biological processes it shares with other genes.


Summary
Cytosolic ribosome-binding translation initiation factor; involved in positive regulation of cytoplasmic translational elongation through polyproline stretches

View computational annotations

Molecular Function

Manually Curated

Cellular Component

Manually Curated
Phenotype Details

Phenotype

Phenotype annotations for a gene are curated single mutant phenotypes that require an observable (e.g., "cell shape"), a qualifier (e.g., "abnormal"), a mutant type (e.g., null), strain background, and a reference. In addition, annotations are classified as classical genetics or high-throughput (e.g., large scale survey, systematic mutation set). Whenever possible, allele information and additional details are provided. Click "Phenotype Details" to view all phenotype annotations and evidence for this locus as well as phenotypes it shares with other genes.


Summary
Essential gene in S288C; conditional (ts) mutations lead to accumulation of polysomes, abnormal actin cytoskeleton and defects in shmoo formation; null mutant is reported as viable but slow growing in some strain backgrounds
Disease Details

Disease

Disease Annotations consist of three mandatory components: a gene product, a term from the Disease Ontology (DO) controlled vocabulary and an evidence code. SGD provides manually curated DO Annotations derived from the literature. Click "Disease Details" to view all Disease information and evidence for this locus as well as diseases it shares with other genes.


Summary
Yeast HYP2 is homologous to human EIF5A and has been used to study how SUMOylation modulates eIF5A activities in pancreatic ductal adenocarcinoma cells
Interaction Details

Interaction

Interaction annotations are curated by BioGRID and include physical or genetic interactions observed between at least two genes. An interaction annotation is composed of the interaction type, name of the interactor, assay type (e.g., Two-Hybrid), annotation type (e.g., manual or high-throughput), and a reference, as well as other experimental details. Click "Interaction Details" to view all interaction annotations and evidence for this locus, including an interaction visualization.


Summary
The hyp2 null mutant is inviable in the S288C and W303 backgrounds, but viable in others; the null mutant of paralog anb1 is viable; the hyp2 anb1 double mutant is inviable.

1230 total interactions for 1081 unique genes

Physical Interactions

  • Affinity Capture-MS: 129
  • Affinity Capture-RNA: 14
  • Affinity Capture-Western: 11
  • Co-fractionation: 1
  • Co-purification: 2
  • PCA: 3
  • Protein-RNA: 1
  • Proximity Label-MS: 1
  • Reconstituted Complex: 2
  • Two-hybrid: 4

Genetic Interactions

  • Dosage Rescue: 30
  • Negative Genetic: 639
  • Phenotypic Suppression: 1
  • Positive Genetic: 375
  • Synthetic Growth Defect: 5
  • Synthetic Lethality: 6
  • Synthetic Rescue: 6
Regulation Details

Regulation

The number of putative Regulators (genes that regulate it) and Targets (genes it regulates) for the given locus, based on experimental evidence. This evidence includes data generated through high-throughput techniques. Click "Regulation Details" to view all regulation annotations, shared GO enrichment among regulation Targets, and a regulator/target diagram for the locus.


Regulators
13
Targets
0
Expression Details

Expression

Expression data are derived from records contained in the Gene Expression Omnibus (GEO), and are first log2 transformed and normalized. Referenced datasets may contain one or more condition(s), and as a result there may be a greater number of conditions than datasets represented in a single clickable histogram bar. The histogram division at 0.0 separates the down-regulated (green) conditions and datasets from those that are up-regulated (red). Click "Expression Details" to view all expression annotations and details for this locus, including a visualization of genes that share a similar expression pattern.


Summary Paragraph

A summary of the locus, written by SGD Biocurators following a thorough review of the literature. Links to gene names and curated GO terms are included within the Summary Paragraphs.


Last Updated: 2009-08-31

Literature Details

Literature

All manually curated literature for the specified gene, organized into topics according to their relevance to the gene (Primary Literature, Additional Literature, or Review). Click "Literature Details" to view all literature information for this locus, including shared literature between genes.


Primary
58
Additional
77
Reviews
15

Resources